Salatin Sara, Maleki Dizaj Solmaz, Yari Khosroushahi Ahmad
Biotechnology Research Center, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
Cell Biol Int. 2015 Aug;39(8):881-90. doi: 10.1002/cbin.10459. Epub 2015 Apr 29.
Nowadays successful application of nanoparticles for therapeutic objects needs the effective uptake of them by cells. Hence, studying of the interaction of nanoparticles with cell membrane for effective cellular uptaking seems to be vital and important. Trafficking of lipids, proteins, glucose, and other biomaterials into the cells is possible from two major exocytic and endocytic pathways. The penetration ability of nanoparticles into the cells must be considered in engineering of these particles. Enormous in vivo and in vitro experiments in the field of nanotechnology have confirmed the effect of physiochemistry properties in state of cell-nanoparticles interactions. Thus, the optimization of parameters directly related to physicochemical characteristics through the preparation process seems to be necessary for improving therapeutic effects of nanocarriers. Besides, biological medium and cell division also affect the amount of nanoparticle uptaking into the cells. This study reviews the influence of size, shape, the surface modification of nano particles, medium, and cell division effects on the cellular absorption of drug/gene nanocarriers.
如今，纳米颗粒在治疗领域的成功应用需要细胞对其有效摄取。因此，研究纳米颗粒与细胞膜的相互作用以实现有效的细胞摄取似乎至关重要。脂质、蛋白质、葡萄糖和其他生物材料通过两种主要的胞吐和胞吞途径进入细胞。在设计这些纳米颗粒时，必须考虑其进入细胞的穿透能力。纳米技术领域大量的体内和体外实验证实了物理化学性质在细胞与纳米颗粒相互作用状态中的作用。因此，通过制备过程优化与物理化学特性直接相关的参数似乎对于提高纳米载体的治疗效果是必要的。此外，生物介质和细胞分裂也会影响纳米颗粒进入细胞的摄取量。本研究综述了尺寸、形状、纳米颗粒的表面修饰、介质以及细胞分裂对药物/基因纳米载体细胞吸收的影响。
