Pratt-Terpstra I H, Weerkamp A H, Busscher H J
Dental School, University of Groningen, The Netherlands.
J Gen Microbiol. 1987 Nov;133(11):3199-206. doi: 10.1099/00221287-133-11-3199.
A flow cell system was developed which allowed the study of bacterial adhesion to solid substrata at well-defined shear rates. In addition, the system enabled the solid surfaces to be coated with a proteinaceous film under exactly the same shear conditions. In this flow cell system, adhesion of three strains of oral streptococci from a phosphate-buffered solution onto three different substrata was studied as a function of time in the absence and presence of a bovine serum albumin (BSA) coating at a shear rate of 21 s-1. To obtain a wide range in surface free energies (gamma) representative strains (gamma b 38-117 mJ m-2) and solid substrata (gamma s 20-109 mJ m-2) were selected. The number of bacteria adhering was counted microscopically. In the absence of a BSA coating a linear relation was found between the number of bacteria adhering at saturation (nb,s) and the calculated interfacial free energy of adhesion (delta Fadh) for each of the three strains. In the presence of a BSA coating the number of bacteria adhering was greatly decreased in all cases. However, despite the presence of the BSA coating there was still a linear relation between the number of bacteria adhering at saturation and the interfacial free energy of adhesion, calculated on the basis of the surface free energy of the uncoated substrata. It can be concluded that the bare, uncoated substratum still influenced bacterial adhesion in spite of the marked influence of a BSA coating.
开发了一种流动池系统，该系统可在明确的剪切速率下研究细菌对固体基质的粘附。此外，该系统能够在完全相同的剪切条件下在固体表面涂上一层蛋白质膜。在这个流动池系统中，研究了来自磷酸盐缓冲溶液的三种口腔链球菌菌株在不存在和存在牛血清白蛋白（BSA）涂层的情况下，在21 s-1的剪切速率下对三种不同基质的粘附随时间的变化。为了获得广泛的表面自由能（γ）范围，选择了代表性菌株（γb 38 - 117 mJ m-2）和固体基质（γs 20 - 109 mJ m-2）。通过显微镜计数粘附的细菌数量。在没有BSA涂层的情况下，发现三种菌株中每种菌株在饱和时粘附的细菌数量（nb,s）与计算得出的粘附界面自由能（ΔFadh）之间存在线性关系。在有BSA涂层的情况下，所有情况下粘附的细菌数量都大大减少。然而，尽管存在BSA涂层，但在饱和时粘附的细菌数量与基于未涂层基质的表面自由能计算得出的粘附界面自由能之间仍然存在线性关系。可以得出结论，尽管BSA涂层有显著影响，但裸露的、未涂层的基质仍然影响细菌粘附。
