Hahnel Sebastian, Rosentritt Martin, Handel Gerhard, Bürgers Ralf
Department of Prosthetic Dentistry, Regensburg University Medical Center, Regensburg, 93042, Germany.
J Mater Sci Mater Med. 2009 Jan;20(1):249-55. doi: 10.1007/s10856-008-3570-7. Epub 2008 Aug 30.
The aim of this in vitro study was to examine potential changes and influences of prosthetic resin surface properties on Candida albicans adhesion after surface treatment or artificial ageing.
Standardized specimens of a denture base resin and a veneering composite were prepared, polished, and randomly subjected to different surface roughness treatments or artificial ageing protocols (storage in ethanol or artificial saliva for 7/90 d, thermocycling). Surface roughness (Ra) and surface free energy were determined prior and after each treatment. Specimens were incubated with phosphate buffered saline or whole saliva for 2 h at 37 degrees C, and later with Candida albicans suspension (2.5 h, 37 degrees C). Adherent viable fungi were quantified using a bioluminescence assay.
Artifical ageing did not affect substratum surface roughness, yet slight increases in substratum surface free energy and significant increases in Candida albicans adhesion were observed. Saliva coating marginally influenced Candida albicans adherence to reference and surface treated specimens, yet more pronounced differences in Candida albicans adhesion between the various artificially aged specimens were found.
No correlation between substratum surface roughness or surface free energy and Candida albicans adhesion could be established.
本体外研究旨在探讨表面处理或人工老化后，义齿修复树脂表面特性对白色念珠菌黏附的潜在变化及影响。
制备义齿基托树脂和饰面复合材料的标准化试件，进行抛光处理，并随机进行不同的表面粗糙度处理或人工老化方案（在乙醇或人工唾液中储存7/90天、热循环）。在每次处理前后测定表面粗糙度（Ra）和表面自由能。将试件在37℃下用磷酸盐缓冲盐水或全唾液孵育2小时，随后用白色念珠菌悬液孵育（2.5小时，37℃）。使用生物发光测定法定量黏附的活真菌。
人工老化未影响基底表面粗糙度，但观察到基底表面自由能略有增加，白色念珠菌黏附显著增加。唾液包被对白色念珠菌黏附于对照和表面处理试件的影响较小，但在不同人工老化试件之间发现白色念珠菌黏附存在更明显差异。
无法确定基底表面粗糙度或表面自由能与白色念珠菌黏附之间的相关性。
