Vidács Dániel László, Veréb Zoltán, Bozó Renáta, Flink Lili Borbála, Polyánka Hilda, Németh István Balázs, Póliska Szilárd, Papp Benjamin Tamás, Manczinger Máté, Gáspár Róbert, Mirdamadi Seyedmohsen, Kemény Lajos, Bata-Csörgő Zsuzsanna
Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.
Hungarian Centre of Excellence for Molecular Medicine - University of Szeged Skin Research Group (HCEMM-USZ Skin Research Group), Szeged, Hungary.
Pigment Cell Melanoma Res. 2022 Jan;35(1):38-51. doi: 10.1111/pcmr.13012. Epub 2021 Aug 31.
We previously described a novel in vitro culture technique for dedifferentiated human adult skin melanocytes. Melanocytes cultured in a defined, cholera toxin and PMA free medium became bipolar, unpigmented, and highly proliferative. Furthermore, TRP-1 and c-Kit expression disappeared and EGFR receptor and nestin expression were induced in the cells. Here, we further characterized the phenotype of these dedifferentiated cells and by comparing them to mature pigmented melanocytes we detected crucial steps in their phenotype change. Our data suggest that normal adult melanocytes easily dedifferentiate into pluripotent stem cells given the right environment. This dedifferentiation process described here for normal melanocyte is very similar to what has been described for melanoma cells, indicating that phenotype switching driven by environmental factors is a general characteristic of melanocytes that can occur independent of malignant transformation.
我们之前描述了一种用于人成年皮肤黑色素细胞去分化的新型体外培养技术。在特定的、不含霍乱毒素和佛波酯的培养基中培养的黑色素细胞变成双极的、无色素的且高度增殖。此外，细胞中的TRP - 1和c - Kit表达消失，而EGFR受体和巢蛋白表达被诱导。在这里，我们进一步对这些去分化细胞的表型进行了表征，并通过将它们与成熟的色素沉着黑色素细胞进行比较，检测到了它们表型变化中的关键步骤。我们的数据表明，在合适的环境下，正常成年黑色素细胞很容易去分化为多能干细胞。这里描述的正常黑色素细胞的去分化过程与黑色素瘤细胞的情况非常相似，表明由环境因素驱动的表型转换是黑色素细胞的一个普遍特征，它可以独立于恶性转化而发生。
