基于长链非编码 RNA 介导的竞争性内源性 RNA 网络鉴定膜性肾病的生物标志物。
Biomarker Identification in Membranous Nephropathy Using a Long Non-coding RNA-Mediated Competitive Endogenous RNA Network.
机构信息
Department of Nephrology, China-Japan Union Hospital of Jilin University, No. 126, Xian Tai Street, Changchun, 130033, Jilin, China.
Department of Emergency, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.
出版信息
Interdiscip Sci. 2021 Dec;13(4):615-623. doi: 10.1007/s12539-021-00466-z. Epub 2021 Sep 1.
PURPOSE
This study was aimed to identify biomarker associated with membranous nephropathy (MN) progression by integration of expression profiles and competitive endogenous RNA (ceRNA) network analysis.
METHODS
The gene (GSE108113) and microRNAs (miRNAs) expression profiles (GSE64306) were downloaded to identify the differentially expressed mRNAs, miRNAs and long non-coding RNAs (lncRNAs) between MN and control groups. The functions and pathways enriched by the differentially expressed mRNAs were analyzed. The mRNA-lncRNA co-expression network was constructed followed by and the ceRNA network construction.
RESULTS
Total 264 upregulated and 196 downregulated differentially expressed mRNAs, 79 upregulated and 4 downregulated lncRNAs, as well as 115 upregulated and 93 downregulated miRNAs were obtained between MN and control groups. After analysis, the differential mRNAs were significantly involved in multiple immune-related processes, and cell proliferation, apoptosis and differentiation processes, as well as pathways of taste transduction and lysosome. Finally, a ceRNA network consisting of 4 mRNAs (EPB41L5, FAM43A, PRKG1 and TTC14), 3 lncRNAs (LINC00052, LINC00641 and N4BP2L2-IT2) and 5 miRNAs (hsa-miR-145-5p, hsa-miR-3605-5p, hsa-miR-148a-3p, hsa-miR-497-5p and hsa-miR-148b-3p) was constructed.
CONCLUSION
Our study indicated dysregulation of immune- and apoptosis-associated functions and taste transduction and lysosome pathways may play important roles in MN progression. Deregulated ceRNAs, such as LINC00052-hsa-miR-145-5p-EPB41L5, LINC00052-hsa-miR-148a-3p-FAM43A and LINC00641-hsa-497-5p-PRKG1, may be associated with MN development.
目的
本研究旨在通过整合表达谱和竞争性内源 RNA（ceRNA）网络分析，鉴定与膜性肾病（MN）进展相关的生物标志物。
方法
下载基因（GSE108113）和 microRNAs（miRNAs）表达谱（GSE64306），以鉴定 MN 组与对照组之间差异表达的 mRNAs、miRNAs 和长非编码 RNA（lncRNAs）。分析差异表达 mRNAs 富集的功能和通路。构建 mRNA-lncRNA 共表达网络，然后构建 ceRNA 网络。
结果
MN 组与对照组间共获得 264 个上调和 196 个下调差异表达 mRNAs、79 个上调和 4 个下调 lncRNAs、115 个上调和 93 个下调 miRNAs。进一步分析发现，差异表达的 mRNAs 显著参与多种免疫相关过程、细胞增殖、凋亡和分化过程以及味觉转导和溶酶体途径。最终构建了一个由 4 个 mRNAs（EPB41L5、FAM43A、PRKG1 和 TTC14）、3 个 lncRNAs（LINC00052、LINC00641 和 N4BP2L2-IT2）和 5 个 miRNAs（hsa-miR-145-5p、hsa-miR-3605-5p、hsa-miR-148a-3p、hsa-miR-497-5p 和 hsa-miR-148b-3p）组成的 ceRNA 网络。
结论
本研究表明，免疫和凋亡相关功能以及味觉转导和溶酶体途径的失调可能在 MN 进展中发挥重要作用。失调的 ceRNAs，如 LINC00052-hsa-miR-145-5p-EPB41L5、LINC00052-hsa-miR-148a-3p-FAM43A 和 LINC00641-hsa-miR-497-5p-PRKG1，可能与 MN 的发生有关。
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