Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, 510642, China.
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, 510642, China.
Fish Shellfish Immunol. 2021 Nov;118:72-84. doi: 10.1016/j.fsi.2021.08.018. Epub 2021 Aug 30.
Misgurnus anguillicaudatus (M. anguillicaudatus) is a widely cultivated fish. However, in M. anguillicaudatus breeding, the frequent cold stress during daily breeding could induce immune suppression and increase the risk of infection, causing serious economic loss. Based on existing findings, CpG Oligonucleotides (CpG-ODNs) may be an ideal protective agent for low temperature fish breeding, performing anti-infective when faced with cold stress with cold shock proteins Y box binding proteins (YBX). Although YBX has pleiotropic functions, its roles in CpG-ODNs-mediated immunity (especially under cold situations) remain largely unexplored. To clarify the relationship among them, we identified the YBX1/YBX2 in M. anguillicaudatus and analyzed using a series of bioinformatics methods. After that, we immunized the fish with 3 types of CpG-ODNs and challenged with Aeromonas hydrophila (A. hydrophila). Here we showed that the best anti-bacterial effect of CpG-B was accompanied by the significant upregulation of YBX1. And the detection of the YBX1 downstream effectors confirmed that CpG-B induced the YBX1-mediated Th1 oriented responses to A. hydrophila by regulation of the NLRP3 (Caspase-A/-B), IL-1β, IL-12 and IFN-γ. Afterwards, we found that under cold stress, CpG-B can activate the NLRP3 and NF-κB pathways through YBX1, a key mediator of anti-A. hydrophila in CpG-B immunization. In this study, we demonstrated CpG-B protection against infection in low temperature, and its interaction with YBX1, expanded the research of CpG-ODN under cold stress, and provided a new CpG-ODN application for low temperature fish farming.
泥鳅（Misgurnus anguillicaudatus）是一种广泛养殖的鱼类。然而，在泥鳅养殖过程中，日常养殖中频繁的冷应激会导致免疫抑制，增加感染风险，造成严重的经济损失。基于现有研究，CpG 寡核苷酸（CpG-ODNs）可能是低温鱼类养殖的理想保护剂，在面临冷应激时与冷休克蛋白 Y 盒结合蛋白（YBX）一起具有抗感染作用。尽管 YBX 具有多种功能，但它在 CpG-ODN 介导的免疫（尤其是在低温情况下）中的作用在很大程度上仍未得到探索。为了阐明它们之间的关系，我们在泥鳅中鉴定了 YBX1/YBX2，并使用一系列生物信息学方法进行了分析。之后，我们用 3 种 CpG-ODN 对鱼进行免疫，并用水生栖热菌（Aeromonas hydrophila，A. hydrophila）进行攻毒。结果表明，CpG-B 的最佳抗菌效果伴随着 YBX1 的显著上调。对 YBX1 下游效应物的检测证实，CpG-B 通过调节 NLRP3（Caspase-A/-B）、IL-1β、IL-12 和 IFN-γ，诱导 YBX1 介导的 Th1 定向抗 A. hydrophila 反应。随后，我们发现，在冷应激下，CpG-B 可以通过关键介质 YBX1 激活 NLRP3 和 NF-κB 通路，从而在 CpG-B 免疫中发挥抗 A. hydrophila 的作用。在这项研究中，我们证明了 CpG-B 可以在低温下抵抗感染，以及它与 YBX1 的相互作用，扩展了 CpG-ODN 在低温下的研究，并为低温水产养殖提供了一种新的 CpG-ODN 应用。
