Naqvi S, Nadvi I N, Zaidi Z H
H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.
Biosci Rep. 1987 Oct;7(10):813-9. doi: 10.1007/BF01116755.
Hemoglobin from the cobra snake, Naja naja naja, was isolated and its chains separated on a CM-cellulose column. The separation profile revealed an alpha and two beta chains having the molar proportions of [alpha]2[beta 1]1[beta 2]1. The N-terminal amino acid sequence of the intact chains and of the CNBr peptides were carried out. The beta 2 chain was found to be heterogeneous comprising a minor component amounting to 11%. This later showed changes at two positions 9 and 14 in the first 30 residues sequenced.
