Roos F J, Zimmermann A, Keller H U
Institute of Pathology, University of Bern, Switzerland.
J Cell Sci. 1987 Oct;88 ( Pt 3):399-406. doi: 10.1242/jcs.88.3.399.
The results show that the distinct types of shape produced by phorbol myristate acetate (PMA) and by chemotactic peptides (fNLPNTL) are associated with distinct types of neutrophil movement. Whereas the chemotactic peptide can induce front-tail polarity characterized by an expanding front, a contracted tail and preferential unidirectional movements of intracellular organelles, PMA can only elicit non-polar movements characterized by random formation and retraction of projections all over the surface, intracellular movements of organelles being ill-defined and changing in direction. Combined stimulation of human neutrophils with PMA and fNLPNTL results in a suppression of peptide-induced polarity and the formation of non-polar motile cells resembling those stimulated with PMA alone. The results suggest that the diacylglycerol-protein kinase C pathway may be instrumental in transducing or modulating signals to the locomotor apparatus of the cell. PMA-treated cells are, however, still capable of developing directional responses when appropriately stimulated. The findings lead to the hypothesis that distinct types of neutrophil movements are preferentially associated with distinct functions.
结果表明，佛波酯（PMA）和趋化肽（fNLPNTL）产生的不同类型的形态与中性粒细胞的不同运动类型相关。趋化肽可诱导前端-尾部极性，其特征为前端扩展、尾部收缩以及细胞内细胞器的优先单向运动，而PMA只能引发非极性运动，其特征为在整个表面随机形成和缩回突起，细胞器的细胞内运动不明确且方向多变。用PMA和fNLPNTL联合刺激人中性粒细胞会导致肽诱导的极性受到抑制，并形成类似于仅用PMA刺激的非极性运动细胞。结果表明，二酰基甘油-蛋白激酶C途径可能有助于将信号转导或调节至细胞的运动装置。然而，PMA处理的细胞在受到适当刺激时仍能够产生定向反应。这些发现导致这样一种假设，即不同类型的中性粒细胞运动优先与不同功能相关。
