Hu Xiaoyu, Shen Xin, Tian Jiangxue
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, China; Department of orthodontics, School of Stomatology, Fourth Military Medical University, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.
Biochem Biophys Res Commun. 2021 Oct 22;576:80-85. doi: 10.1016/j.bbrc.2021.07.092. Epub 2021 Aug 5.
Epidemiological data have shown that periodontal bacterial infection, periodontitis, and oral squamous cell carcinoma have close relationship on the disease progress and risk. However, the specific role of periodontal microbes and their mechanism in the development of oral squamous cell carcinoma is not yet clear. In our previous work, metagenomic Illumina Mi-seq analysis was used to identify tstructure and abundance of periodontital microbiome. Accoding to the results, we used Porphyromonas.spp. and Fusobacterium.spp. as the periodontitis positive microbiota; Neisseria.spp and Corynebacterium.spp as periodontitis negative microbiota (their average relative abundance were >5%). These representative strains of the above genus were used to infect OSCC cells to explore their effect on tumor cell biology behavior, and detect the expression level of the gene in related to inflammation, migration, invasion and cell cycle. We find that periodontitis positive correlated microbiota had a promoting effect on the development of oral squamous cell carcinoma in vitro by regulating mRNA and protein expression of IL-6, IL-8, MMP-9 and Cyclin-D1. Periodontitis negative correlated microbiota had suppression effect on the development of oral squamous cell carcinoma in vitro analysis.
流行病学数据表明，牙周细菌感染、牙周炎与口腔鳞状细胞癌在疾病进展和风险方面存在密切关系。然而，牙周微生物在口腔鳞状细胞癌发生发展中的具体作用及其机制尚不清楚。在我们之前的工作中，利用宏基因组Illumina Mi-seq分析来鉴定牙周微生物群的结构和丰度。根据结果，我们将卟啉单胞菌属和梭杆菌属作为牙周炎阳性微生物群；奈瑟菌属和棒状杆菌属作为牙周炎阴性微生物群（它们的平均相对丰度>5%）。用上述属的这些代表性菌株感染口腔鳞状细胞癌细胞，以探讨它们对肿瘤细胞生物学行为的影响，并检测与炎症、迁移、侵袭和细胞周期相关基因的表达水平。我们发现，牙周炎正相关微生物群通过调节IL-6、IL-8、MMP-9和细胞周期蛋白D1的mRNA和蛋白表达，在体外对口腔鳞状细胞癌的发展具有促进作用。体外分析显示，牙周炎负相关微生物群对口腔鳞状细胞癌的发展具有抑制作用。
