Crop Development Centre, Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.
Plant Genome. 2021 Nov;14(3):e20131. doi: 10.1002/tpg2.20131. Epub 2021 Sep 5.
Anthracnose, caused byColletotrichum lentis, is a devastating disease of lentil (Lens culinaris Medik.) in western Canada. Growing resistant lentil cultivars is the most cost-effective and environmentally friendly approach to prevent seed yield losses that can exceed 70%. To identify loci conferring resistance to anthracnose race 1 in lentil, biparental quantitative trait loci (QTL) mapping of two recombinant inbred line (RIL) populations was integrated with a genome-wide association study (GWAS) using 200 diverse lentil accessions from a lentil diversity panel. A major-effect QTL (qAnt1.Lc-3) conferring resistance to race 1 was mapped to lentil chromosome 3 and colocated on the lentil physical map for both RIL populations. Clusters of candidate nucleotide-binding leucine-rich repeat (NB-LRR) and other defense-related genes were uncovered within the QTL region. A GWAS detected 14 significant single nucleotide polymorphism (SNP) markers associated with race 1 resistance on chromosomes 3, 4, 5, and 6. The most significant GWAS SNPs on chromosome 3 supported qAnt1.Lc-3 and delineated a region of 1.6 Mb containing candidate resistance genes. The identified SNP markers can be directly applied in marker-assisted selection (MAS) to accelerate the introgression of race 1 resistance in lentil breeding.
炭疽病由炭疽菌引起，是加拿大西部小扁豆（Lens culinaris Medik.）的一种毁灭性疾病。培育抗炭疽病的小扁豆品种是防止种子产量损失的最具成本效益和环保的方法，损失可超过 70%。为了鉴定小扁豆中对炭疽病 1 号菌系具有抗性的基因座，对来自小扁豆多样性面板的 200 个不同小扁豆品系的两个重组自交系（RIL）群体进行了双亲数量性状基因座（QTL）作图，并与全基因组关联研究（GWAS）相结合。一个赋予小扁豆 1 号菌系抗性的主效 QTL（qAnt1.Lc-3）被定位到小扁豆 3 号染色体上，并在两个 RIL 群体的小扁豆物理图谱上共定位。在 QTL 区域内发现了候选核苷酸结合亮氨酸重复（NB-LRR）和其他防御相关基因的簇。GWAS 在染色体 3、4、5 和 6 上检测到与 1 号菌系抗性相关的 14 个显著单核苷酸多态性（SNP）标记。染色体 3 上最显著的 GWAS SNP 支持 qAnt1.Lc-3，并描绘了一个包含候选抗性基因的 1.6 Mb 区域。鉴定出的 SNP 标记可直接应用于标记辅助选择（MAS），以加速小扁豆育种中对 1 号菌系抗性的基因导入。
