Department of Horticulture, Washington State University, Pullman, WA 99164, USA.
Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA.
Int J Mol Sci. 2020 Mar 20;21(6):2129. doi: 10.3390/ijms21062129.
Lentil ( Medikus) is an important source of protein for people in developing countries. Aphanomyces root rot (ARR) has emerged as one of the most devastating diseases affecting lentil production. In this study, we applied two complementary quantitative trait loci (QTL) analysis approaches to unravel the genetic architecture underlying this complex trait. A recombinant inbred line (RIL) population and an association mapping population were genotyped using genotyping by sequencing (GBS) to discover novel single nucleotide polymorphisms (SNPs). QTL mapping identified 19 QTL associated with ARR resistance, while association mapping detected 38 QTL and highlighted accumulation of favorable haplotypes in most of the resistant accessions. Seven QTL clusters were discovered on six chromosomes, and 15 putative genes were identified within the QTL clusters. To validate QTL mapping and genome-wide association study (GWAS) results, expression analysis of five selected genes was conducted on partially resistant and susceptible accessions. Three of the genes were differentially expressed at early stages of infection, two of which may be associated with ARR resistance. Our findings provide valuable insight into the genetic control of ARR, and genetic and genomic resources developed here can be used to accelerate development of lentil cultivars with high levels of partial resistance to ARR.
兵豆（Medikus）是发展中国家人们获取蛋白质的重要来源。根肿菌根腐病（ARR）已成为影响兵豆生产的最具破坏性疾病之一。在这项研究中，我们应用了两种互补的数量性状位点（QTL）分析方法来揭示这个复杂性状的遗传结构。重组自交系（RIL）群体和关联作图群体使用测序基因型（GBS）进行了基因型分析，以发现新的单核苷酸多态性（SNP）。QTL 作图确定了 19 个与 ARR 抗性相关的 QTL，而关联作图检测到 38 个 QTL，并强调了大多数抗性品系中有利单倍型的积累。在六个染色体上发现了七个 QTL 簇，在 QTL 簇内鉴定出了 15 个推定基因。为了验证 QTL 作图和全基因组关联研究（GWAS）的结果，对五个选定基因在部分抗性和易感品系上进行了表达分析。三个基因在感染早期表现出差异表达，其中两个可能与 ARR 抗性有关。我们的研究结果为 ARR 的遗传控制提供了有价值的见解，并且这里开发的遗传和基因组资源可用于加速具有高水平部分抗性的 ARR 的兵豆品种的开发。
