Shanxi Provincial People's Hospital, Taiyuan, China.
Sixth Hospital of Shanxi Medical University, Taiyuan, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211039110. doi: 10.1177/15330338211039110.
Our study aimed to evaluate the correlation of circular RNA SMARCA5 (circ-SMARCA5) and microRNA 432 (miR-432) with clinical characteristics and survival in bladder cancer patients. Preoperative clinicopathologic features and survival data of 156 bladder cancer patients were retrospectively reviewed. A total of 156 cases of tumor tissues, whereas 71 cases out of 156 available adjacent tissues were obtained from the Pathology Department for circ-SMARCA5 and miR-432 detections using real-time quantitative polymerase chain reaction. Circ-SMARCA5 was upregulated but miR-432 was downregulated in tumor tissues compared with adjacent tissues; meanwhile, circ-SMARCA5 expression was negatively correlated with miR-432 in bladder cancer tissues. Circ-SMARCA5 high expression was correlated with larger tumor size, higher tumor stage, and lymph node (LYN) metastasis. However, miR-432 high expression was correlated with single multiplicity, smaller tumor size, lower tumor stage, less LYN metastasis in bladder cancer patients. Regarding survival, circ-SMARCA5 high expression was correlated with shorter disease-free survival (DFS) and overall survival (OS); whereas, miR-432 high expression was correlated with longer DFS and OS in bladder cancer patients. Further multivariate Cox's regression analysis displayed that circ-SMARCA5 high expression was an independent predictive factor for both worse DFS and OS in bladder cancer patients. Circ-SMARCA5 high expression but miR-432 low expression is correlated with advanced tumor features and poor survival of bladder cancer patients, which present as potential prognostic markers in bladder cancer.
我们的研究旨在评估环状 RNA SMARCA5（circ-SMARCA5）和 microRNA 432（miR-432）与膀胱癌患者临床特征和生存的相关性。回顾性分析了 156 例膀胱癌患者的术前临床病理特征和生存数据。从病理科获得了 156 例肿瘤组织和 156 例可获得的相邻组织中的 71 例，用于使用实时定量聚合酶链反应检测 circ-SMARCA5 和 miR-432。与相邻组织相比，肿瘤组织中 circ-SMARCA5 上调，但 miR-432 下调；同时，circ-SMARCA5 表达与膀胱癌组织中的 miR-432 呈负相关。circ-SMARCA5 高表达与肿瘤较大、肿瘤分期较高和淋巴结（LYN）转移有关。然而，miR-432 高表达与膀胱癌患者的单一多发性、肿瘤较小、肿瘤分期较低、LYN 转移较少有关。就生存而言，circ-SMARCA5 高表达与无病生存（DFS）和总生存（OS）较短相关；而 miR-432 高表达与膀胱癌患者的 DFS 和 OS 较长相关。进一步的多变量 Cox 回归分析显示，circ-SMARCA5 高表达是膀胱癌患者 DFS 和 OS 较差的独立预测因子。circ-SMARCA5 高表达而 miR-432 低表达与膀胱癌患者的晚期肿瘤特征和不良生存相关，它们是膀胱癌潜在的预后标志物。
