Department of Oncology, The First Affiliated Hospital, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, China.
Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, NHC Key Laboratory of Liver Transplantation, Nanjing, Jiangsu, China.
Dis Markers. 2019 Mar 6;2019:2473652. doi: 10.1155/2019/2473652. eCollection 2019.
Circular RNAs have been implicated in various malignancies and can function as potential biomarkers for cancers. Reportedly, circSMARCA5 was downregulated in hepatocellular carcinoma and glioblastoma multiforme, but upregulated in prostate cancer. The functional roles and clinical significance of circSMARCA5 still remain unknown in the context of gastric cancer (GC).
Expression levels of circSMARCA5 were detected by qRT-PCR. Clinical data including patient basic information, clinicopathological features, and survival data were obtained. The Kaplan-Meier methods, multivariate Cox regression models, and the receiver operating characteristic curve were used to assess the clinical significance of circSMARCA5 in GC. Cell proliferation assays and transwell assays were performed to elucidate the functional roles of circSMARCA5 in GC.
The circSMARCA5 level was decreased in GC tissues and cell lines. The low expression level of circSMARCA5 was correlated to poorer overall survival and disease-free survival. Low circSMARCA5 expression was revealed as an independent unfavorable predictive factor for GC. The results indicated that circSMARCA5 had a moderate ability for discrimination between GC patients and controls with an area under the curve of 0.806. Upregulation of circSMARCA5 dampened the proliferation, migration, and invasion of GC cells, whereas circSMARCA5 knockdown promoted GC progression.
Our results demonstrated that circSMARCA5 was decreased and exerted tumor-suppressive effects in GC. circSMARCA5 can function as a potential biomarker for GC prognosis and diagnosis.
环状 RNA 已被牵涉到多种恶性肿瘤中，并可作为癌症的潜在生物标志物。据报道，circSMARCA5 在肝细胞癌和多形性胶质母细胞瘤中下调，但在前列腺癌中上调。circSMARCA5 在胃癌（GC）中的功能作用和临床意义尚不清楚。
通过 qRT-PCR 检测 circSMARCA5 的表达水平。获取包括患者基本信息、临床病理特征和生存数据在内的临床数据。使用 Kaplan-Meier 方法、多变量 Cox 回归模型和受试者工作特征曲线评估 circSMARCA5 在 GC 中的临床意义。进行细胞增殖实验和 Transwell 实验以阐明 circSMARCA5 在 GC 中的功能作用。
circSMARCA5 的水平在 GC 组织和细胞系中降低。circSMARCA5 的低表达水平与总体生存率和无病生存率较差相关。circSMARCA5 低表达被揭示为 GC 的独立不利预后因素。结果表明，circSMARCA5 在区分 GC 患者和对照组方面具有中等的区分能力，曲线下面积为 0.806。circSMARCA5 的上调抑制了 GC 细胞的增殖、迁移和侵袭，而 circSMARCA5 的下调则促进了 GC 的进展。
我们的结果表明，circSMARCA5 在 GC 中减少并发挥肿瘤抑制作用。circSMARCA5 可作为 GC 预后和诊断的潜在生物标志物。
