Shaltiel-Harpaz Liora, Yahyaa Mosaab, Nawade Bhagwat, Dudareva Natalia, Ibdah Mwafaq
Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel; Tel Hai College, Environmental Sciences Department, Upper Galilee, 12210, Israel.
Newe Yaar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel.
Plant Sci. 2021 Oct;311:111011. doi: 10.1016/j.plantsci.2021.111011. Epub 2021 Aug 2.
Carrot psylla is one of the devastating pests of carrot throughout northern Europe and the Mediterranean basin. Here we characterized the behavioral response of psylla females towards different carrot germplasm and identified the chemical cues involved in the host selection of psylla females by oviposition choice experiments and metabolic profiling of leaf volatiles. In choice assays, carrot psylla displayed differential responses to tested 14 germplasm. Among germplasm, wild accessions 21793 and 20465 were highly preferred by carrot psylla, while wild accessions 20465 and the orange cultivar Nairobi were less. In non-choice experiments conducted only with this four-germplasm revealed that the carrot psylla females gave higher preference to the Nairobi and wild accession 20465, indicating the vicinity to other host plants in the same area might affect female preference. Moreover, the nymph development and survival experiments showed the lowest nymphs survival rate on the wild accessions 21793 and 20497. Furthermore, the volatile emissions among different carrot cultivars infested with psylla showed qualitative and quantitative differences versus intact plants. Among these volatiles, apiol, β-asarone, myristicin, and sabinene showed a relationship with psyllas growth and survival. We also showed that myristicin and sabinene exogenous applications caused a dramatic reduction in the number of eggs laid by psylla and subsequent nymph survival. This is an initial study of the volatiles that mediate attraction and oviposition preference of carrot psylla in response to its host plant. The results from this study provide baseline information for the development of new control strategies against carrot psylla.
胡萝卜木虱是北欧和地中海盆地胡萝卜的毁灭性害虫之一。在此，我们通过产卵选择实验和叶片挥发物代谢谱分析，对木虱雌虫对不同胡萝卜种质的行为反应进行了表征，并确定了参与木虱雌虫寄主选择的化学线索。在选择试验中，胡萝卜木虱对测试的14种种质表现出不同的反应。在种质中，野生种质21793和20465是胡萝卜木虱高度偏好的，而野生种质20465和橙色品种内罗毕则较不受欢迎。仅对这四种种质进行的非选择实验表明，胡萝卜木虱雌虫对内罗毕和野生种质20465有更高的偏好，这表明同一区域内其他寄主植物的存在可能会影响雌虫的偏好。此外，若虫发育和存活实验表明，在野生种质21793和20497上若虫存活率最低。此外，与未受侵染的完整植株相比，受木虱侵染的不同胡萝卜品种的挥发物排放表现出质和量的差异。在这些挥发物中，芹菜脑、β-细辛醚、肉豆蔻醚和桧烯与木虱的生长和存活有关。我们还表明，外源施用肉豆蔻醚和桧烯会导致木虱产卵数量和随后若虫存活率大幅下降。这是关于介导胡萝卜木虱对其寄主植物产生吸引和产卵偏好的挥发物的初步研究。本研究结果为开发针对胡萝卜木虱的新控制策略提供了基线信息。
