Manuel Kevin, Ambroise Marie Moses, Ramdas Anita, Varghese Renu G'Boy
Department of Pathology, Pondicherry Institute of Medical Sciences, Kalapet, Puducherry, India.
J Lab Physicians. 2021 Jun;13(2):156-161. doi: 10.1055/s-0041-1730849. Epub 2021 Jun 19.
Proper serological testing for the definite diagnosis of dengue is costly and may not be easily available in a resource-limited setting. Hematological parameters can help in the early identification of dengue cases. This study aims to evaluate the occurrence and utility of pseudobasophilia in identifying dengue-affected patients. This retrospective cross-sectional study included 1,304 dengue cases confirmed by serology and 1,044 dengue serology negative acute febrile illness cases as controls. Complete blood count (CBC) values of the first EDTA (ethylenediamine tetraacetic acid) blood sample from automated hematology analyzers were reviewed. The hematological parameters in the dengue and control groups were compared and the variation of these parameters with the day of fever was also analyzed. Mann-Whitney's test, Kruskal-Wallis test, and Fisher's exact test were used for statistical analysis. A -value < 0.05 was considered statistically significant for all tests. There was a statistically significant variation between dengue cases and controls for hematocrit, platelet count, mean platelet volume, total white blood cell count, and absolute basophil count. The dengue group had a higher hematocrit from day 2 to day 10, platelet count ≤ 100,000/µL from day 4 to day 9, higher mean platelet volume from day 2 to day 7, leucopenia from day 3 to day 5, and higher absolute basophil count from day 2 to day 10. Interestingly, pseudobasophilia was seen in 533 (40.87%) of dengue cases and only 3 (0.28%) of the controls. Pseudobasophilia was also observed to have an increasing trend to the day of fever. Pseudobasophilia along with other CBC parameters is useful and cost effective for the early identification of dengue. This can prompt early investigations and supportive treatment leading to improved clinical outcomes.
进行适当的血清学检测以明确诊断登革热成本高昂，而且在资源有限的环境中可能无法轻易获得。血液学参数有助于早期识别登革热病例。本研究旨在评估假性嗜碱性粒细胞增多症在识别登革热感染患者中的发生情况及其效用。
这项回顾性横断面研究纳入了1304例经血清学确诊的登革热病例和1044例登革热血清学阴性的急性发热性疾病病例作为对照。回顾了自动血液分析仪检测的第一份乙二胺四乙酸（EDTA）血样的全血细胞计数（CBC）值。比较了登革热组和对照组的血液学参数，并分析了这些参数随发热天数的变化。
采用曼-惠特尼检验、克鲁斯卡尔-沃利斯检验和费舍尔精确检验进行统计分析。所有检验中，P值<0.05被认为具有统计学意义。
登革热病例与对照组在血细胞比容、血小板计数、平均血小板体积、总白细胞计数和绝对嗜碱性粒细胞计数方面存在统计学显著差异。登革热组在第2天至第10天血细胞比容较高，第4天至第9天血小板计数≤100,000/µL，第2天至第7天平均血小板体积较高，第3天至第5天白细胞减少，第2天至第10天绝对嗜碱性粒细胞计数较高。有趣的是，533例（40.87%）登革热病例出现假性嗜碱性粒细胞增多症，而对照组仅3例（0.
