Blood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital, Ullevaal, Oslo, Norway.
Am J Clin Pathol. 2012 Jan;137(1):86-92. doi: 10.1309/AJCP19BFTHYTMORO.
We compared the performance of the basophil count of 3 hematology instruments with a flow cytometric method (FCM) in which CD123 and CD193 were used as basophil markers. By analyzing 112 patient samples, we found the ADVIA 120 (Siemens Healthcare Diagnostics, Deerfield, IL) and CELL-DYN Sapphire (Abbott Diagnostics, Santa Clara, CA) to underestimate the number of basophils by approximately 50% and the Sysmex XE-2100 (Sysmex, Kobe, Japan) and ADVIA to overestimate the basophil count in some samples with pathologic leukocytes. All 3 instruments had large (25%-50%) analytic within-run coefficients of variation. Compared with the FCM, we found a relatively good correlation for the CELL-DYN basophil count (r = 0.81), an intermediate correlation for the Sysmex (r = 0.64), and a poor correlation for the ADVIA (r = 0.24). When excluding the 52 samples flagged for the presence of pathologic leukocytes, these correlations were found to be 0.84, 0.90, and 0.57, respectively. The basophil count of the 3 instruments is, at least presently, of unsatisfactory quality.
我们比较了三种血液学仪器的嗜碱性粒细胞计数与使用 CD123 和 CD193 作为嗜碱性粒细胞标志物的流式细胞术（FCM）方法的性能。通过分析 112 个患者样本，我们发现 ADVIA 120（西门子医疗诊断，迪尔菲尔德，IL）和 CELL-DYN Sapphire（雅培诊断，圣克拉拉，CA）大约低估了 50%的嗜碱性粒细胞数量，而 Sysmex XE-2100（希森美康，神户，日本）和 ADVIA 在一些病态白细胞的样本中高估了嗜碱性粒细胞计数。所有 3 种仪器的分析内重复性变异系数都很大（25%-50%）。与 FCM 相比，我们发现 CELL-DYN 的嗜碱性粒细胞计数相关性较好（r = 0.81），Sysmex 的相关性中等（r = 0.64），ADVIA 的相关性较差（r = 0.24）。当排除 52 个标记有病态白细胞的样本时，这些相关性分别为 0.84、0.90 和 0.57。至少目前来看，这三种仪器的嗜碱性粒细胞计数质量并不令人满意。
