氧化锌/铜-壳聚糖/蒙脱石的抗菌活性及作用机制
Antibacterial Activity and Mechanism of ZnO/Cu-Chitosan/Montmorillonite.
作者信息
Ma Xiaoxia, Gao Lingjuan, Ma Yulong, Zhang Xiaoguang
机构信息
State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, College of Chemistry and Chemical Engineering, Ningxia University, Yinchuan, 750021 China.
出版信息
J Wuhan Univ Technol Mater Sci Ed. 2021;36(4):510-516. doi: 10.1007/s11595-021-2438-2. Epub 2021 Aug 29.
A new composite antibacterial material ZnO/Cu-Chitosan/Montmorillonite (ZCCM) was prepared with montmorillonite as carrier, Zn(Ac)•2HO, Cu(NO)•3HO and chitosan as raw materials. ZCCM was characterized by X-ray diffraction, nitrogen physical adsorption, scanning electron microscopy and energy dispersion spectrometry. The antibacterial activity of ZCCM against , and was evaluated by minimal inhibitory concentration, minimum bactericidal concentration and the influence of growth curves. ZCCM displays excellent antibacterial activity which is higher than ZnO-Montmorillonite, Cu-Montmorillonite and ZnO/Cu-Montmorillonite. In addition, the antibacterial mechanism of ZCCM was investigated by analyzing bacterial morphology, integrity of cell membrane, lipid peroxidation and the effect of histidine on antibacterial activity of materials. It is found that cell morphologies of bacteria are damaged and bacterial cells are shrunken. With the increase of cell membrane permeability, the intracellular dissolved matters leak continuously. What's more, the reactive oxygen species are generated and biomacromolecules are oxidized.
以蒙脱石为载体，以Zn(Ac)•2HO、Cu(NO)•3HO和壳聚糖为原料制备了一种新型复合抗菌材料ZnO/Cu-壳聚糖/蒙脱石（ZCCM）。采用X射线衍射、氮物理吸附、扫描电子显微镜和能谱分析对ZCCM进行了表征。通过最低抑菌浓度、最低杀菌浓度和生长曲线的影响来评价ZCCM对 、 和 的抗菌活性。ZCCM表现出优异的抗菌活性，高于ZnO-蒙脱石、Cu-蒙脱石和ZnO/Cu-蒙脱石。此外，通过分析细菌形态、细胞膜完整性、脂质过氧化以及组氨酸对材料抗菌活性的影响，研究了ZCCM的抗菌机制。结果发现，细菌的细胞形态受到破坏，细菌细胞萎缩。随着细胞膜通透性的增加，细胞内溶解物质不断泄漏。此外，还产生了活性氧并氧化了生物大分子。
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