Pan Jianheng, Cao Zhanhong, Fang Chunqiu, Lei Yuting, Sun Jiaming, Huang Xiaowei, Han Dong
Department of Pharmacy, Changchun University of Chinese Medicine, Changchun, China.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China.
Front Pharmacol. 2021 Aug 13;12:722530. doi: 10.3389/fphar.2021.722530. eCollection 2021.
Myocardial fibrosis (MF) is an important pathological process in which a variety of cardiovascular diseases transform into heart failure. The main manifestation of MF is the excessive deposition of collagen in the myocardium. Here, we explored whether Huangqi Shengmai Yin (HSY) can inhibit isoprenaline (ISO)-induced myocardial collagen deposition in rats, thereby reducing the cardiac dysfunction caused by MF. The results of echocardiography showed that HSY upregulated fractional shortening and ejection fraction, and reduced the left ventricular systolic dysfunction in the rats with MF. Pathological results showed that HSY protected myocardium, inhibited apoptosis, and effectively reduced collagen deposition. HSY also inhibited the expression of collagen I and III and α-smooth muscle actin (α-SMA) in the heart tissue. HSY increased the expression of Sirtuin 3 (Sirt3) and inhibited the protein levels of the components in the transforming growth factor-β (TGF-β)/Smad pathway. At the same time, it also regulated the expression of related proteins in the matrix metalloproteinases family. In summary, HSY played a therapeutic role in rats with ISO-induced MF by protecting myocardium and inhibiting collagen deposition. Therefore, HSY is a potential therapeutic agent for ameliorating MF.
心肌纤维化（MF）是多种心血管疾病转变为心力衰竭的重要病理过程。MF的主要表现是心肌中胶原蛋白过度沉积。在此，我们探讨了黄芪生脉饮（HSY）是否能抑制异丙肾上腺素（ISO）诱导的大鼠心肌胶原沉积，从而减轻MF所致的心功能障碍。超声心动图结果显示，HSY上调了缩短分数和射血分数，并减轻了MF大鼠的左心室收缩功能障碍。病理结果表明，HSY保护心肌，抑制细胞凋亡，并有效减少胶原沉积。HSY还抑制心脏组织中I型和III型胶原蛋白以及α-平滑肌肌动蛋白（α-SMA）的表达。HSY增加了沉默调节蛋白3（Sirt3）的表达，并抑制了转化生长因子-β（TGF-β）/Smad通路中各组分的蛋白水平。同时，它还调节基质金属蛋白酶家族相关蛋白的表达。综上所述，HSY通过保护心肌和抑制胶原沉积对ISO诱导的MF大鼠发挥治疗作用。因此，HSY是改善MF的一种潜在治疗药物。
