Department of General Surgery, Yueqing People's Hospital, 338 Qingyuan road, Yueqing, 325600 Zhejiang Province, China.
Key Lab of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, 101 Longmian Avenue, Nanjing 211166, China.
Dis Markers. 2021 Aug 25;2021:7253633. doi: 10.1155/2021/7253633. eCollection 2021.
Gastrointestinal (GI) cancers are among the most fatal diseases in the world. Numerous studies have demonstrated the relationship between autophagy and development of gastrointestinal cancers. However, whether autophagy-related genes can predict prognosis of GI cancers in individuals of Asian ancestry has not been defined. This study, evaluated the prognostic value of autophagy-related genes in gastrointestinal cancer. Expression profile of autophagy-related genes for 296 gastrointestinal cancer patients of Asian ancestry was downloaded from the TCGA database (TCGA-LIHC, TCGA-STAD, TCGA-ESCA, TCGA-PAAD, TCGA-COAD, TCGA-CHOL, and TCGA-READ). The prognostic value of the autophagy-related genes was evaluated using univariate Cox, LASSO, and multivariate Cox regression analyses. The risk score of the autophagy-related gene signature was calculated to assess its predictive prognostic value for GI cancers. Forty-seven differentially expressed autophagy-related genes, in Asian patients with gastrointestinal cancers, were identified. Of the 47 genes, 4 were associated with prognosis of GI cancer (SQSTM1, BIRC5, NRG3, and CXCR4). A prognostic model for GI cancer, based on the expression of the above 4 genes in the training set, showed that cancer patients were stratified into high-risk and low-risk groups ( < 0.05). The utility of the model for overall survival (OS) of GI cancer patients was consistent across the entire set, training set, and test set (entire set: = 4.568 × 10; train set: = 5.718 × 10; test set: = 3.516 × 10). The sensitivity and specificity of the ROC curve of the above prognostic model in predicting the 5-year prognosis of GI cancer was satisfactory (entire set: 0.728; train set: 0.727; test set: 0.733). Analysis of clinical samples validated the overexpression of the 4 genes (SQSTM1, BIRC5, NRG3, and CXCR4) in tumor tissues relative to paired normal tissues, consistent with bioinformatic findings. Expression of the 4 autophagy-related genes (SQSTM1, BIRC5, NRG3, and CXCR4) can accurately predict the prognosis of gastrointestinal tumors in Asian patients.
胃肠道（GI）癌症是世界上最致命的疾病之一。许多研究已经证明了自噬与胃肠道癌症发展之间的关系。然而，自噬相关基因是否可以预测亚洲血统的 GI 癌症患者的预后尚未确定。本研究评估了自噬相关基因在胃肠道癌症中的预后价值。从 TCGA 数据库（TCGA-LIHC、TCGA-STAD、TCGA-ESCA、TCGA-PAAD、TCGA-COAD、TCGA-CHOL 和 TCGA-READ）下载了 296 名亚洲胃肠道癌症患者的自噬相关基因表达谱。使用单因素 Cox、LASSO 和多因素 Cox 回归分析评估自噬相关基因的预后价值。计算自噬相关基因特征的风险评分，以评估其对 GI 癌症的预测预后价值。确定了 47 个差异表达的自噬相关基因，在亚洲胃肠道癌症患者中。在这 47 个基因中，有 4 个与 GI 癌症的预后相关（SQSTM1、BIRC5、NRG3 和 CXCR4）。基于上述 4 个基因在训练集中的表达，建立了一个 GI 癌症的预后模型，结果表明癌症患者被分为高风险和低风险组（<0.05）。该模型对 GI 癌症患者总生存率（OS）的预测在整个数据集、训练集和测试集均一致（整个数据集：=4.568×10；训练集：=5.718×10；测试集：=3.516×10）。上述预后模型预测 GI 癌症 5 年预后的 ROC 曲线的灵敏度和特异性令人满意（整个数据集：0.728；训练集：0.727；测试集：0.733）。临床样本分析验证了 4 个基因（SQSTM1、BIRC5、NRG3 和 CXCR4）在肿瘤组织中的表达高于配对的正常组织，与生物信息学结果一致。4 个自噬相关基因（SQSTM1、BIRC5、NRG3 和 CXCR4）的表达可以准确预测亚洲患者胃肠道肿瘤的预后。
