Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
Department of Urology, Guangzhou Institute of Urology, Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510230, China.
Oxid Med Cell Longev. 2021 Aug 26;2021:5555796. doi: 10.1155/2021/5555796. eCollection 2021.
The original polysaccharide (LP0) was sulfated using the sulfur trioxide-pyridine method, and four sulfated polysaccharides (SLPs) were obtained, namely, SLP1, SLP2, SLP3, and SLP4. The sulfated (-OSO ) contents were 8.58%, 15.1%, 22.8%, and 31.3%, respectively. The structures of the polysaccharides were characterized using a Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance (NMR) techniques. SLPs showed better antioxidant activity than LP0, increased the concentration of soluble Ca in the solution, reduced the amount of CaOx precipitation and degree of CaOx crystal aggregation, induced COD crystal formation, and protected HK-2 cells from damage caused by nanometer calcium oxalate crystals. These effects can inhibit the formation of CaOx kidney stones. The biological activity of the polysaccharides increased with the content of -OSO , that is, the biological activities of the polysaccharides had the following order: LP0 < SLP1 < SLP2 < SLP3 < SLP4. These results reveal that SLPs with high -OSO contents are potential drugs for effectively inhibiting the formation of CaOx stones.
原始多糖（LP0）采用三氧化硫-吡啶法进行硫酸化处理，得到四种硫酸化多糖（SLP），分别为 SLP1、SLP2、SLP3 和 SLP4。硫酸化（-OSO ）含量分别为 8.58%、15.1%、22.8%和 31.3%。采用傅里叶变换红外（FT-IR）光谱仪和核磁共振（NMR）技术对多糖的结构进行了表征。SLP 比 LP0 具有更好的抗氧化活性，增加了溶液中可溶性 Ca 的浓度，减少了 CaOx 沉淀量和 CaOx 晶体聚集度，诱导 COD 晶体形成，并保护 HK-2 细胞免受纳米草酸钙晶体损伤。这些作用可以抑制 CaOx 肾结石的形成。多糖的生物活性随 -OSO 含量的增加而增加，即多糖的生物活性具有以下顺序：LP0 < SLP1 < SLP2 < SLP3 < SLP4。这些结果表明，具有高 -OSO 含量的 SLP 是有效抑制 CaOx 结石形成的潜在药物。
