Department of Pharmacy, Central University ‟Marta Abreu de Las Villas", Cuba.
Chemical Bioactive Center, Central University ‟Marta Abreu of Las Villas", Cuba.
J Ethnopharmacol. 2020 May 10;253:112691. doi: 10.1016/j.jep.2020.112691. Epub 2020 Feb 21.
Boldoa purpurascens Cav. (Nyctaginaceae) is a plant species used in traditional medicine in Cuba as antiurolithiatic.
The aim of the present investigation was to evaluate the in vitro and in vivo antiurolothiatic activity of an aqueous extract from the leaves of Boldoa purpurascens.
The aqueous extract from leaves of Boldoa purpurascens was evaluated for antiurolithiatic activity in vitro and in vivo. In vitro crystallization of calcium oxalate (CaOx) was assessed using a nucleation, aggregation and growth assay. The effects of the extract and of Cystone®, used as a positive control, on the slope of nucleation and aggregation, as well as on the growth of CaOx crystals, were evaluated spectrophotometrically. The densities of the formed crystals were compared microscopically. In vivo activity was evaluated in an urolithiasis model in rats, in which kidney stones are induced by ethylene glycol (0.75%) and ammonium chloride (2%) in drinking water for 10 days. Three different experimental doses (100, 200 and 400 mg/kg, p.o.) of the extract and Cystone® were administered for 10 days. After 10 days, various biochemical parameters were measured in urine and serum, and histopathological analysis of the kidneys was carried out.
The aqueous extract of Boldoa purpurascens inhibited the slope of nucleation and aggregation of CaOx crystallization, and decreased the crystal density. It also inhibited the growth and caused the dissolution of CaOx crystals. Cystone® exhibited similar effects. At a dose of 400 mg/kg the extract reduced the concentration of uric acid in urine, as well as the serum concentration of uric acid and creatinine. Histopathologic analysis of the kidneys of the same treatment group revealed reduced tissue damage; the results were almost similar to the untreated healthy control group.
This study indicates that an aqueous leaf extract of Boldoa purpurascens may be effective in the prevention of urinary stone formation, and substantiates the traditional claim.
Boldoa purpurascens Cav.（紫茉莉科）是古巴传统医学中用于抗尿路结石的植物物种。
本研究旨在评估 Boldoa purpurascens 叶的水提物的体外和体内抗尿路结石活性。
评估 Boldoa purpurascens 叶的水提物的体外和体内抗尿路结石活性。使用成核、聚集和生长测定法评估体外草酸钙 (CaOx) 的结晶。通过分光光度法评估提取物和 Cystone®（用作阳性对照）对成核和聚集斜率以及 CaOx 晶体生长的影响。比较显微镜下形成的晶体的密度。在大鼠尿石症模型中评估体内活性，在该模型中，通过在饮用水中添加乙二醇（0.75%）和氯化铵（2%）10 天来诱导肾结石。给予提取物和 Cystone®三个不同的实验剂量（100、200 和 400 mg/kg，po），共 10 天。10 天后，测量尿液和血清中的各种生化参数，并对肾脏进行组织病理学分析。
Boldoa purpurascens 的水提物抑制了 CaOx 结晶成核和聚集斜率，并降低了晶体密度。它还抑制了生长并导致 CaOx 晶体溶解。Cystone®也表现出类似的作用。在 400mg/kg 剂量下，提取物降低了尿液中尿酸的浓度，以及血清中尿酸和肌酐的浓度。同一治疗组的肾脏组织病理学分析显示组织损伤减少；结果几乎与未经治疗的健康对照组相似。
本研究表明，Boldoa purpurascens 的叶水提物可能有效预防尿路结石形成，并证实了传统说法。
