Pak J Biol Sci. 2021 Jan;24(8):881-887. doi: 10.3923/pjbs.2021.881.887.
<b>Background and Objective:</b> <i>Cordyceps militaris </i>is a potential edible medicinal mushroom which containing various biological activity such as anti-inflammatory, anti-ageing, anti-protozoal and anti-microbial. The compositions of <i>C. militaris</i> media were composed of carbon source, nitrogen source and other additives. This research aimed to evaluate the effect of edible insects on the <i>C. militaris </i>mycelium formation. <b>Materials and Methods:</b> Seven edible insects including <i>Bombyx mori </i>L., <i>Samia ricini</i> D., <i>Acheta domesticus</i> L., <i>Gryllus bimaculatus</i> De Geer, <i>Tenebrio molitor</i> L., <i>Rhynchophorus ferrugineus</i> and <i>Lethocerus indicus</i> were used as nitrogen source supplemented in Potato Dextrose Agar (PDA) and the mycelium formation of each edible insects at day 7, 14 and 21 were recorded. <b>Results:</b> The results of nitrogen source from a boiled edible insect at day 21 indicated that the highest colony diameter at 88.00 mm was obtained when cultured with PDA+<i>B. mori</i> L. The results of nitrogen source from a dried edible insect at day 21 presented that the highest diameter at 84.33 mm was obtained from cultured using PDA+<i>A. domesticus</i> L. <b>Conclusion:</b> The suitable boiled and dried edible insects for the supplement in PDA were boiled <i>B. mori</i> L. and dried <i>A. domesticus </i>L. This is the first report about PDA supplemented with edible insects that can be increased the <i>C. militaris</i> mycelium formation which the initial stage that important for <i>C. militaris </i>cultivation.
蛹虫草是一种具有多种生物活性的潜在可食用药用真菌,具有抗炎、抗衰老、抗原生动物和抗微生物作用。蛹虫草培养基的组成包括碳源、氮源和其他添加剂。本研究旨在评估食用昆虫对蛹虫草菌丝体形成的影响。材料与方法:选用 7 种食用昆虫,包括家蚕(Bombyx mori L.)、蓖麻蚕(Samia ricini D.)、东亚飞蝗(Acheta domesticus L.)、蝼蛄(Gryllus bimaculatus De Geer)、黄粉虫(Tenebrio molitor L.)、红火蚁(Rhynchophorus ferrugineus)和红火蚁(Lethocerus indicus)作为氮源添加到马铃薯葡萄糖琼脂(PDA)中,并记录第 7、14 和 21 天每种食用昆虫的菌丝体形成情况。结果:21 天的煮沸食用昆虫氮源结果表明,在 PDA+家蚕(Bombyx mori L.)中培养时,菌落直径最大,为 88.00mm。21 天的干燥食用昆虫氮源结果表明,在 PDA+东亚飞蝗(Acheta domesticus L.)中培养时,直径最大,为 84.33mm。结论:适合在 PDA 中添加的煮沸和干燥食用昆虫是煮沸的家蚕(Bombyx mori L.)和干燥的东亚飞蝗(Acheta domesticus L.)。这是首次报道在 PDA 中添加食用昆虫可以增加蛹虫草菌丝体的形成,这是蛹虫草培养的重要初始阶段。