Turk Ayman, Abdelhamid Mohamed A A, Yeon Sang Won, Ryu Se Hwan, Lee Solip, Ko Sung Min, Kim Beom Seok, Pack Seung Pil, Hwang Bang Yeon, Lee Mi Kyeong
College of Pharmacy, Chungbuk National University, Cheongju, South Korea.
Department of Biotechnology and Bioinformatics, Korea University, Sejong, South Korea.
Front Microbiol. 2022 Oct 19;13:1017576. doi: 10.3389/fmicb.2022.1017576. eCollection 2022.
Cordycepin is the major constituent of mushroom (or ) with therapeutic potential. Insects are the direct sources of nutrients for in nature. Therefore, optimized condition of cultivation for efficient cordycepin production was explored using six edible insects as substrates. The highest yield of cordycepin was produced by the cultivation on and was 34 times that on pupae. Among insect components, fat content was found to be important for cordycepin production. Especially, a positive correlation was deduced between oleic acid content and cordycepin production. The transcriptional levels of and , genes involved in cordycepin biosynthesis, were higher in grown on than on other insects tested. The addition of oleic acid to the substrates increased cordycepin production together with the transcriptional levels of and . Therefore, with high content of cordycepin can be secured by the cultivation on insects.
虫草素是具有治疗潜力的蘑菇（或 ）的主要成分。在自然界中，昆虫是 的直接营养来源。因此，以六种食用昆虫为底物，探索了优化 培养条件以高效生产虫草素。以 为底物培养时虫草素产量最高，是 蛹培养产量的34倍。在昆虫成分中，脂肪含量对虫草素生产很重要。特别是，油酸含量与虫草素生产之间存在正相关。参与虫草素生物合成的 和 基因的转录水平，在以 为底物生长的 中比在其他测试昆虫中更高。向底物中添加油酸可增加虫草素产量以及 和 的转录水平。因此，通过在昆虫上培养可以确保获得高含量虫草素的 。
