Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, China.
Department of Dermatology, Huangshi Central Hospital of Edong Healthcare Group, Hubei Polytechnic University, Huangshi, Hubei, China.
Histol Histopathol. 2021 Sep;36(9):995-1005. doi: 10.14670/HH-18-372. Epub 2021 Aug 23.
Long noncoding RNAs (lncRNAs) are the most recently discovered class of noncoding RNAs. LncRNAs play a crucial role in multiple disorders. However, the role and mechanism of action of lncRNAs in keloids remain unclear. Here, qRT-PCR and western blotting assays were performed to determine the expression of genes and proteins, respectively. MTT assays were carried out to measure the proliferation of keloid fibroblasts. In addition, a luciferase activity assay was conducted to investigate the relationships between LINC00937 and miR-28-5p and between miR-28-5p and MC1R. The results showed that LINC00937 and MC1R were decreased, whereas miR-28-5p was increased in keloid tissues. LINC00937 overexpression in keloid fibroblasts could repress the extracellular matrix (ECM) deposition and cell proliferation and promote MC1R expression. Moreover, high expression of miR-28-5p and low expression of LINC00937 were detected in keloid fibroblasts. We further showed that LINC00937 promoted MC1R expression by sponging miR-28-5p. Finally, our data indicated that LINC00937 inhibited the ECM deposition and proliferation of keloid fibroblasts by inhibiting miR-28-5p and facilitating MC1R expression. Overall, LINC00937 suppressed the ECM deposition and proliferation of keloid fibroblasts by acting as an miR-28-5p sponge and promoting MC1R expression. Our data suggested that LINC00937 is a potential target for keloid treatment.
长链非编码 RNA（lncRNAs）是最近发现的一类非编码 RNA。lncRNAs 在多种疾病中发挥着关键作用。然而，lncRNAs 在瘢痕疙瘩中的作用和作用机制尚不清楚。在这里，分别进行了 qRT-PCR 和 Western blot 测定以确定基因和蛋白质的表达。进行了 MTT 测定以测量瘢痕疙瘩成纤维细胞的增殖。此外，进行了荧光素酶活性测定以研究 LINC00937 与 miR-28-5p 之间以及 miR-28-5p 与 MC1R 之间的关系。结果表明，LINC00937 和 MC1R 减少，而 miR-28-5p 在瘢痕疙瘩组织中增加。LINC00937 在瘢痕疙瘩成纤维细胞中的过表达可抑制细胞外基质（ECM）沉积和细胞增殖，并促进 MC1R 表达。此外，在瘢痕疙瘩成纤维细胞中检测到 miR-28-5p 高表达和 LINC00937 低表达。我们进一步表明，LINC00937 通过海绵吸附 miR-28-5p 促进 MC1R 表达。最后，我们的数据表明，LINC00937 通过抑制 miR-28-5p 并促进 MC1R 表达来抑制 ECM 沉积和瘢痕疙瘩成纤维细胞的增殖。总体而言，LINC00937 通过充当 miR-28-5p 海绵并促进 MC1R 表达来抑制瘢痕疙瘩成纤维细胞的 ECM 沉积和增殖。我们的数据表明，LINC00937 是瘢痕疙瘩治疗的潜在靶标。
