Department of Biomedical Engineering, Kocaeli University, Kocaeli, Turkey.
Department of Bioengineering, University of California - Los Angeles, Los Angeles, California, USA.
J Drug Target. 2022 Feb;30(2):188-199. doi: 10.1080/1061186X.2021.1971235. Epub 2021 Sep 6.
Sustained external supply of oxygen (O) to engineered tissue constructs is important for their survival in the body while angiogenesis is taking place. In the recent years, the trend towards the fabrication of various O-generating materials that can provide prolonged and controlled O source to the large volume tissue constructs resulted in preventing necrosis associated with the lack of O supply. In this review, we explain different methods employed in the fabrication of O-generating materials such as emulsion, microfluidics, solvent casting, freeze drying, electrospraying, gelation, microfluidic and three-dimensional (3D) bioprinting methods. After discussing pros and cons of each method, we review physical, chemical, and biological characterisation techniques used to analyse the resulting product. Finally, the challenges and future directions in the field are discussed.
为了使工程化组织构建体在血管生成过程中存活，持续向其外部供应氧气（O）非常重要。近年来，人们致力于制造各种能够为大容量组织构建体提供长时间和可控的 O 源的产氧材料，以防止因 O 供应不足而导致的坏死。在这篇综述中，我们解释了制造产氧材料的不同方法，如乳液、微流控、溶剂浇铸、冷冻干燥、静电纺丝、凝胶化、微流控和三维（3D）生物打印方法。在讨论了每种方法的优缺点之后，我们还回顾了用于分析最终产物的物理、化学和生物学特性分析技术。最后，讨论了该领域的挑战和未来发展方向。
