Institute for Animal Hygiene, Animal Welfare and Farm Animal Behavior, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Clin Hemorheol Microcirc. 2021;79(1):149-155. doi: 10.3233/CH-219201.
The pig is the most widely used large animal model in Europe, with cardiovascular research being one of the main areas of application. Adequate refinement of interventional studies in this field, meeting the requirements of Russel and Burchs' 3 R concept, can only be performed if blood-contacting medical devices are hemocompatible. Because most medical devices for cardiovascular interventional procedures are developed for humans they are tested mostly for compatibility with human blood. The aim of this study was therefore to determine whether there are differences in behavior of porcine and human platelets when they come into contact with glass, which was used as an exemplary thrombogenic material. For this purpose changes of platelet count, platelet volume and platelet expression of the activation markers CD61, CD62P and CD63 were measured using a modified chandler loop-system simulating the fluidic effects of the blood flow. Minipig and human platelets showed significant differences in number and volume, but not in activation after 4-8 min exposure to glass.
猪是欧洲应用最广泛的大型动物模型，心血管研究是其主要应用领域之一。只有当与血液接触的医疗器械具有良好的血液相容性时，才能对该领域的介入研究进行充分的改进，满足 Russell 和 Burch 的 3R 概念的要求。因为大多数心血管介入医疗器械都是为人类开发的，所以它们主要测试与人类血液的相容性。因此，本研究旨在确定当猪和人类血小板与用作示例血栓形成材料的玻璃接触时，它们的行为是否存在差异。为此，使用改良的 Chandler 环系统测量血小板计数、血小板体积和血小板活化标志物 CD61、CD62P 和 CD63 的表达，该系统模拟了血流的流体力学效应。在 4-8 分钟暴露于玻璃后，小型猪和人类血小板的数量和体积存在显著差异，但在激活方面没有差异。
