Institute for Animal Hygiene, Animal Welfare and Farm Animal Behavior, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Clin Hemorheol Microcirc. 2021;79(1):129-136. doi: 10.3233/CH-219104.
In human cardiovascular research, sheep in particular are used as a large animal model in addition to pigs. In these animals, medical products, developed and tested for human medical purposes, are almost exclusively used in interventional studies. Therefore, the extent to which platelets from human and ovine blood differ in terms of adherence, aggregation and activation after a 4- or 8-minutes exposure to glass was investigated. Testing was performed with platelet-rich plasma (PRP) and a modified chandler loop-system, with 4- and 8-minute blood-material exposure times corresponding to 20 and 40 test cycles, respectively, through the entire silicone tube loop of the test system.In sheep and human PRP, contact with the silicone tubing resulted in a decrease in platelet count after 4 minutes and 20 test cycles, respectively. Four more minutes (20 additional test cycles) caused a further decrease of the platelet count only in sheep PRP. When the silicon tube was partly filled with glass beads, these effects were more pronounced and stronger in sheep then in human PRP.The mean platelet volume, which was used as parameter for platelet aggregation, did not change over time in human PRP without glass exposure. With glass exposure in human and sheep PRP the mean platelet volume increased within 40 test cycles, but this increase was stronger in sheep than in human PRP.Regarding activation behavior, the activation markers CD62P and CD63 were detectable only in < 30% (sheep) and < 45% (human) of platelets, whereas after 8 min of glass exposure, the proportion of CD62P+ and CD63+ cells was more increased than before only in sheep. These results indicate that ovine platelets adhere more strongly to glass and show stronger aggregation behavior after glass contact than human platelets, but that ovine and human platelets differ only slightly in activability by glass.
在人类心血管研究中，除了猪之外，绵羊还特别被用作大型动物模型。在这些动物中，为人类医疗目的而开发和测试的医疗产品几乎专门用于介入研究。因此，研究了在 4 分钟或 8 分钟暴露于玻璃后，来自人类和绵羊血液的血小板在粘附、聚集和激活方面的差异。使用富含血小板的血浆（PRP）和改良的 Chandler 环系统进行测试，4 分钟和 8 分钟的血液-材料暴露时间分别对应于 20 和 40 个测试循环，通过测试系统的整个硅树脂管环。在绵羊和人类 PRP 中，与硅树脂管接触导致血小板计数在 4 分钟和 20 个测试循环后分别下降。再增加 4 分钟（另外 20 个测试循环）仅导致绵羊 PRP 中的血小板计数进一步下降。当硅管部分填充玻璃珠时，这些效果在绵羊中比在人类 PRP 中更为明显和强烈。平均血小板体积，用作血小板聚集的参数，在人类 PRP 中没有玻璃暴露的情况下随时间没有变化。在人类和绵羊 PRP 中，在玻璃暴露的情况下，平均血小板体积在 40 个测试循环内增加，但绵羊中的增加幅度大于人类 PRP。关于激活行为，只有在绵羊（绵羊）和人类（人类）血小板的 30%（绵羊）和 45%（人类）中才能检测到 CD62P 和 CD63 的激活标记，而在玻璃暴露 8 分钟后，只有绵羊的 CD62P+和 CD63+细胞的比例比暴露前增加更多。这些结果表明，绵羊血小板与玻璃的粘附力更强，与玻璃接触后表现出更强的聚集行为，而绵羊和人类血小板在玻璃激活方面仅略有差异。
