Institute for Animal Hygiene, Animal Welfare and Farm Animal Behavior, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Institute of Biotechnology, Molecular Cell Biology, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany.
Clin Hemorheol Microcirc. 2024;88(s1):S35-S44. doi: 10.3233/CH-248103.
The pig (Sus scrofa) is the most widely used large animal model in Europe, with cardiovascular research being one of the main areas of application. Adequate refinement of interventional studies in this field, meeting the requirements of Russell and Burch's 3 R concept, can only be performed if blood-contacting medical devices are hemocompatible. Because most medical devices for cardiovascular interventional procedures are developed for humans, they are tested only for compatibility with human blood. The aim of this study was therefore to determine whether there are differences in behavior of human and porcine platelets from commercial hybrid pigs when they come into contact with borosilicate glass, which was used as an exemplary thrombogenic material. For this purpose, changes in platelet count, platelet volume and platelet expression of the activation markers CD61, CD62P and CD63 were measured using a modified chandler loop-system simulating the fluidic effects of the bloodflow. Commercial hybrid pig and human platelets showed significant adhesions to borosilicate glass but the commercial hybrid pigs platelets showed a significantly higher tendency to adhere to borosilicate glass. In contrast to human platelets the platelets of commercial hybrid pigs showed significant activation after 4 to 8 minutes exposure to borosilicate glass and there were differences among the ratios of surface and activation markers in between the platelets of both species.
猪（Sus scrofa）是欧洲最广泛使用的大型动物模型，心血管研究是其主要应用领域之一。只有当与血液接触的医疗器械具有血液相容性时，才能充分改进该领域的介入研究，满足 Russell 和 Burch 的 3R 概念的要求。因为大多数心血管介入程序的医疗器械都是为人类开发的，所以只测试它们与人血的相容性。因此，本研究旨在确定当接触硼硅酸盐玻璃时，来自商业杂交猪的人类和猪血小板的行为是否存在差异，硼硅酸盐玻璃被用作典型的血栓形成材料。为此，使用改良的 Chandler 环系统测量血小板计数、血小板体积和激活标志物 CD61、CD62P 和 CD63 的血小板表达的变化，该系统模拟了血流的流体力学效应。商业杂交猪和人血小板对硼硅酸盐玻璃有明显的黏附，但商业杂交猪血小板对硼硅酸盐玻璃的黏附倾向明显更高。与人类血小板不同，商业杂交猪的血小板在接触硼硅酸盐玻璃 4 至 8 分钟后表现出明显的激活，并且两种物种的血小板之间在表面和激活标志物的比值上存在差异。
