Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China.
J Dent. 2021 Oct;113:103799. doi: 10.1016/j.jdent.2021.103799. Epub 2021 Sep 4.
To provide a comprehensive review on the proteomic profile of in vivo acquired enamel pellicle (AEP) formed on permanent teeth.
DATA/SOURCES: PubMed, EMBASE (Ovid) and Web of Science databases were searched for eligible studies (up to December 2020). Studies reporting mass spectrometry-based proteomic analysis of in vivo AEP were included. Risk of bias assessment was performed. Qualitative and quantitative proteomic data were extracted, integrated, then followed by bioinformatic analysis.
Eleven studies were included, involving 122 systemically and dentally healthy adults from four different research groups. Pooled AEP samples from study participants were the normal practice for all included studies. A total of 257/93/108/870 non-redundant proteins were detectable from the in vivo ≤ 5 min/10-min/60-min/2-h AEP samples, respectively. Fifteen "core in vivo 2-h AEP proteins", generally associated with immune and/or inflammatory responses, were consistently identifiable from all four research groups. Eight included studies conducted relative quantitative proteomic analysis, while no statistical analysis could be undertaken due to the inherent limitation of the relative quantification in the proteomics analyses of these studies.
A systematic review on adult in vivo AEP proteomic profile was undertaken. The results provide a comprehensive appreciation of the AEP proteome in healthy individuals from in vivo sampling. Further studies are warranted to clarify the biological role of AEP on oral health, particularly at an individual level.
A comprehensive appreciation of the proteomic profile of in vivo AEP in healthy individuals is essential to further understand its functions in oral health and disease. The information generated also provides insights for future studies.
全面综述体内获得性牙釉质蛋白（AEP）的蛋白质组学特征。
PubMed、EMBASE（Ovid）和 Web of Science 数据库检索了符合条件的研究（截至 2020 年 12 月）。纳入了基于质谱的体内 AEP 蛋白质组学分析的研究。对偏倚风险进行了评估。提取、整合了定性和定量蛋白质组学数据，然后进行了生物信息学分析。
纳入了 11 项研究，涉及来自四个不同研究组的 122 名系统和牙齿健康的成年人。所有纳入研究均采用 pooled AEP 样本。从体内的 AEP 样本中分别可检测到 257/93/108/870 个非冗余蛋白，这些样本的获得时间分别为≤5 分钟/10 分钟/60 分钟/2 小时。15 个“核心体内 2 小时 AEP 蛋白”通常与免疫和/或炎症反应有关，从四个研究组中均能一致地识别出来。8 项纳入的研究进行了相对定量蛋白质组学分析，但由于这些研究中蛋白质组学分析的相对定量存在固有局限性，因此无法进行统计分析。
对成人体内 AEP 蛋白质组学特征进行了系统综述。研究结果全面了解了健康个体体内 AEP 蛋白质组。需要进一步的研究来阐明 AEP 在口腔健康中的生物学作用，特别是在个体水平上。
全面了解健康个体体内 AEP 的蛋白质组学特征对于进一步理解其在口腔健康和疾病中的功能至关重要。所产生的信息也为未来的研究提供了参考。
