Moreira Hugo Mr, Guerra Liberal Francisco Dc, McMahon Stephen J, Prise Kevin M
The Patrick G Johnston Centre for Cancer Research, Queen's University of Belfast, 97 Lisburn Road, Belfast, BT9 7AE, United Kingdom.
The Patrick G Johnston Centre for Cancer Research, Queen's University of Belfast, 97 Lisburn Road, Belfast, BT9 7AE, United Kingdom.
Appl Radiat Isot. 2021 Nov;177:109931. doi: 10.1016/j.apradiso.2021.109931. Epub 2021 Sep 3.
A compact in-house alpha particle source has been developed and fully characterized. The irradiation source is a large area, 25 cm, 5.4 MeV average energy Am source, above which a Mylar dish containing a monolayer of target cells can be placed at defined positions. The source uniformity, flux, particle energy and dose rate were determined experimentally. The dose rate to the nucleus at the closest position was 1.57 Gy/min. Furthermore, a 3D printed collimator was tested and found to improve the uniformity of the energy spectra of particles reaching the target. For validation, prostate PC-3 cells were irradiated in our experimental setup with absorbed doses up to 2 Gy and for reference compared with cells irradiated with conventional X-rays with doses up to 8 Gy. The Relative Biological Effectiveness for alpha particles at 10% survival was 3.66± 0.40 agreeing with previously published data. Data presented here show the feasibility of utilising a low-cost alpha-irradiation source for accurate in vitro assays to better understand the radiobiological effects of high LET alpha particles.
一种紧凑的内部阿尔法粒子源已被开发并进行了全面表征。该辐照源是一个大面积的、直径25厘米、平均能量为5.4兆电子伏特的镅源，在其上方可以将装有单层靶细胞的聚酯薄膜盘放置在规定位置。通过实验确定了源的均匀性、通量、粒子能量和剂量率。最接近位置处细胞核的剂量率为1.57戈瑞/分钟。此外，对一个3D打印的准直器进行了测试，发现它能改善到达靶标的粒子能谱的均匀性。为进行验证，在我们的实验装置中用高达2戈瑞的吸收剂量对前列腺PC-3细胞进行辐照，并与用高达8戈瑞的传统X射线辐照的细胞作为参考进行比较。在10%存活率时阿尔法粒子的相对生物效能为3.66±0.40，与先前发表的数据一致。此处呈现的数据表明，利用低成本的阿尔法辐照源进行精确的体外测定以更好地理解高传能线密度阿尔法粒子的放射生物学效应是可行的。
