Li Yuwen, Li Suhua, Du Ran, Wang Jiaojiao, Li Haiou, Xie Daoxin, Yan Jianbin
Tsinghua-Peking Center for Life Science, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Front Plant Sci. 2021 Aug 19;12:628328. doi: 10.3389/fpls.2021.628328. eCollection 2021.
Amino acids are the building blocks of biomacromolecules in organisms, among which isoleucine (Ile) is the precursor of JA-Ile, an active molecule of phytohormone jasmonate (JA). JA is essential for diverse plant defense responses against biotic and abiotic stresses. is a necrotrophic nutritional fungal pathogen that causes the second most severe plant fungal disease worldwide and infects more than 200 kinds of monocot and dicot plant species. In this study, we demonstrated that Ile application enhances plant resistance against in , which is dependent on the JA receptor COI1 and the jasmonic acid-amido synthetase JAR1. The mutant with higher Ile content in leaves exhibits enhanced resistance to infection. Furthermore, we found that the exogenous Ile application moderately enhanced plant resistance to in various horticultural plant species, including lettuce, rose, and strawberry, suggesting a practical and effective strategy to control disease in agriculture. These results together showed that the increase of Ile could positively regulate the resistance of various plants to by enhancing JA signaling, which would offer potential applications for crop protection.
氨基酸是生物体中生物大分子的组成部分，其中异亮氨酸（Ile）是植物激素茉莉酸（JA）的活性分子茉莉酸异亮氨酸（JA-Ile）的前体。JA对于植物抵御生物和非生物胁迫的多种防御反应至关重要。 是一种坏死营养型真菌病原体，可导致全球第二严重的植物真菌病害，并感染200多种单子叶和双子叶植物物种。在本研究中，我们证明了施用Ile可增强植物对 在 中的抗性，这依赖于JA受体COI1和茉莉酸酰胺合成酶JAR1。叶片中Ile含量较高的突变体 对 感染表现出增强的抗性。此外，我们发现外源施用Ile适度增强了包括生菜、玫瑰和草莓在内的各种园艺植物物种对 的抗性，这表明了一种在农业中控制 病害的实用且有效的策略。这些结果共同表明，Ile的增加可通过增强JA信号传导来正向调节各种植物对 的抗性，这将为作物保护提供潜在应用。
