Ogita Tasuku, Namai Fu, Mikami Ayane, Ishiguro Takahiro, Umezawa Koji, Uyeno Yutaka, Shimosato Takeshi
Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, Japan.
Department of Pathology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
Front Nutr. 2021 Aug 19;8:701466. doi: 10.3389/fnut.2021.701466. eCollection 2021.
The maintenance of intestinal homeostasis is necessary for a good quality of life, and strengthening of the intestinal barrier function is thus an important issue. Therefore, we focused on soybean resistant protein (SRP) derived from (freeze-dried tofu), which is a traditional Japanese food, as a functional food component. In this study, to investigate the effect of SRP on the intestinal barrier function and intestinal microbiota, we conducted an SRP free intake experiment in mice. Results showed that ingestion of SRP decreased the serum level of lipopolysaccharide-binding protein and induced the expression of Reg3γ, thereby improving the intestinal barrier function. In addition, SRP intake induced changes in the cecal microbiota, as observed by changes in β-diversity. In particular, in the microbiota, the up-regulation of functional gene pathways related to the bacterial invasion of epithelial cells (ko05100) was observed, suggesting that Reg3γ expression was induced by the direct stimulation of epithelial cells. The results of this study suggest that SRP is a functional food component that may contribute to the maintenance of intestinal homeostasis.
维持肠道内稳态对于良好的生活质量至关重要，因此增强肠道屏障功能是一个重要问题。因此，我们将源自日本传统食品（冻豆腐）的大豆抗性蛋白（SRP）作为一种功能性食品成分进行了研究。在本研究中，为了探究SRP对肠道屏障功能和肠道微生物群的影响，我们在小鼠中进行了无SRP摄入实验。结果表明，摄入SRP可降低血清脂多糖结合蛋白水平并诱导Reg3γ的表达，从而改善肠道屏障功能。此外，通过β多样性的变化观察到，摄入SRP会引起盲肠微生物群的变化。特别是在微生物群中，观察到与上皮细胞细菌侵袭相关的功能基因途径（ko05100）上调，这表明Reg3γ的表达是由上皮细胞的直接刺激诱导的。本研究结果表明，SRP是一种可能有助于维持肠道内稳态的功能性食品成分。
