Health Science Research Center, Morinaga & Co., Ltd., 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan.
Kanagawa Institute of Industrial Science and Technology, LiSE 4F C-4 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan.
Nutrients. 2020 Feb 11;12(2):449. doi: 10.3390/nu12020449.
Amazake is a traditional Japanese beverage. Its main ingredients are sake cake and rice malt. In this study, we examined the effect of sake cake and rice malt on the intestinal barrier function and gut microbiota. BALB/c mice were fed a control diet or a diet containing a mixture of sake cake and rice malt powder (SRP) for four weeks. Fecal IgA values did not change between groups, but the fecal mucin level was significantly greater in the SRP-fed group. Gene expression analysis in the ileum by real-time PCR demonstrated expression did not change, while the expression was upregulated in the SRP-fed group. Furthermore, microbiota analysis demonstrated a change by SRP intake at the family level, and the proportion of significantly increased in the SRP-fed group. At the genus level, the proportion of also significantly increased in the SRP-fed group. These results suggest that the intake of a mixture of sake cake and rice malt improves intestinal barrier function by increasing mucin levels and inducing changes in intestinal microbiota.
味噌是一种传统的日本饮料。它的主要成分是清酒饼和米曲。在这项研究中，我们研究了清酒饼和米曲对肠道屏障功能和肠道微生物群的影响。BALB/c 小鼠用对照饮食或含有清酒饼和米曲粉混合物的饮食喂养四周。粪便 IgA 值在两组之间没有变化，但 SRP 喂养组的粪便粘蛋白水平显著增加。实时 PCR 对回肠的基因表达分析表明 表达没有变化，而 SRP 喂养组的 表达上调。此外，微生物组分析表明，SRP 摄入在家族水平上发生了变化，并且 SRP 喂养组中的 比例显著增加。在属水平上，SRP 喂养组中的 比例也显著增加。这些结果表明，清酒饼和米曲混合物的摄入通过增加粘蛋白水平和诱导肠道微生物群的变化来改善肠道屏障功能。
