Galzitskaya Oxana V
Laboratory of Bioinformatics and Proteomics, Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia.
Laboratory of the Structure and Function of Muscle Proteins, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Front Mol Biosci. 2021 Aug 19;8:705069. doi: 10.3389/fmolb.2021.705069. eCollection 2021.
Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. The hypothesis of directed co-aggregation of the target protein and an amyloidogenic peptide acting as an antimicrobial peptide was successfully tested for peptides synthesized on the basis of ribosomal S1 protein in the bacterial culture of . Co-aggregation of the target protein and amyloidogenic peptide was also tested for the pathogenic ribosomal S1 protein from . Almost all peptides that we selected as AMPs, prone to aggregation and formation of fibrils, based on the amino acid sequence of ribosomal S1 protein from formed amyloid fibrils. We have demonstrated that amyloidogenic peptides are not only toxic to their target cells, but also some of them have antimicrobial activity. Controlling the aggregation of vital bacterial proteins can become one of the new directions of research and form the basis for the search and development of targeted antibacterial drugs.
抗菌肽（AMPs）及类似化合物是对抗抗生素耐药细菌的潜在候选物。在细菌培养物中，基于核糖体S1蛋白合成的肽成功验证了靶蛋白与作为抗菌肽的淀粉样生成肽定向共聚集的假说。还对来自[具体来源]的致病性核糖体S1蛋白进行了靶蛋白与淀粉样生成肽的共聚集测试。基于来自[具体来源]的核糖体S1蛋白的氨基酸序列，我们选择的几乎所有易于聚集并形成原纤维的作为AMPs的肽都形成了淀粉样原纤维。我们已经证明，淀粉样生成肽不仅对其靶细胞有毒，而且其中一些还具有抗菌活性。控制重要细菌蛋白的聚集可能成为新的研究方向之一，并为靶向抗菌药物的研发奠定基础。
