Gillham N W, Boynton J E, Harris E H
Department of Zoology, Duke University, Durham, NC 27706.
Curr Genet. 1987;12(1):41-7. doi: 10.1007/BF00420726.
Minute colony mutations in C. reinhardtii are induced with 100% efficiency by intercalating dyes such as acriflavin and ethidium bromide. These mutants form small colonies on petri plates because they undergo only 8-9 mitotic divisions before growth ceases. In liquid media without the dye the mutants show gross alterations in mitochondrial structure and function. Here we demonstrate that induction of minute mutations is accompanied by the specific loss of mitochondrial DNA. We also provide evidence that the transmission of the minute colony phenotype in crosses can be explained in terms of uniparental transmission of mitochondrial DNA by the mt- parent.
莱茵衣藻中的微小菌落突变可被吖啶黄素和溴化乙锭等嵌入染料以100%的效率诱导产生。这些突变体在培养皿上形成小菌落，因为它们在生长停止前仅经历8 - 9次有丝分裂。在没有染料的液体培养基中，这些突变体表现出线粒体结构和功能的显著改变。在此我们证明，微小突变的诱导伴随着线粒体DNA的特异性丢失。我们还提供证据表明，在杂交中微小菌落表型的传递可以用线粒体DNA由mt-亲本单亲传递来解释。
