莱茵衣藻中线粒体DNA突变体的诱导与鉴定
Induction and characterization of mitochondrial DNA mutants in Chlamydomonas reinhardtii.
作者信息
Matagne R F, Michel-Wolwertz M R, Munaut C, Duyckaerts C, Sluse F
机构信息
Département de Botanique, Université de Liège, Sart Tilman, Belgium.
出版信息
J Cell Biol. 1989 Apr;108(4):1221-6. doi: 10.1083/jcb.108.4.1221.
Abstract
In addition to lethal minute colony mutations which correspond to loss of mitochondrial DNA, acriflavin induces in Chlamydomonas reinhardtii a low percentage of cells that grow in the light but do not divide under heterotrophic conditions. Two such obligate photoautotrophic mutants were shown to lack the cyanide-sensitive cytochrome pathway of the respiration and to have a reduced cytochrome c oxidase activity. In crosses to wild type, the mutations are transmitted almost exclusively from the mating type minus parent. A same pattern of inheritance is seen for the mitochondrial DNA in crosses between the two interfertile species C. reinhardtii and Chlamydomonas smithii. Both mutants have a deletion in the region of the mitochondrial DNA containing the apocytochrome b gene and possibly the unidentified URFx gene.
摘要
除了与线粒体DNA缺失相对应的致死性微小菌落突变外，吖啶黄素还能在莱茵衣藻中诱导出一小部分细胞，这些细胞在光照下生长，但在异养条件下不分裂。已证明两个这样的专性光合自养突变体缺乏呼吸作用的氰化物敏感型细胞色素途径，并且细胞色素c氧化酶活性降低。在与野生型杂交时，这些突变几乎只从负交配型亲本传递。在两个可育的物种莱茵衣藻和史密斯衣藻杂交中，线粒体DNA也呈现相同的遗传模式。两个突变体在线粒体DNA中含有脱辅基细胞色素b基因以及可能未鉴定的URFx基因的区域都有缺失。
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