Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
J Hazard Mater. 2021 Aug 15;416:126053. doi: 10.1016/j.jhazmat.2021.126053. Epub 2021 May 13.
Various genotoxic substances in household effluents have not been sufficiently studied. The purpose of this study is to evaluate them using the umu test after dividing them based on the acid-base properties of their functional groups by solid-phase extraction cartridges. The results of the samples concentrated with reverse-phase cartridges showed that the substances with acid functional groups had stronger genotoxicity as 4.1-12.1 ng-4-NQO/mL without S9 enzyme and 17.4-51.8 ng-2-AA/mL with S9 enzyme, while the basic substances also showed a certain degree of toxicity. The results of dividing the effluents by acid-base properties using ion-exchange cartridges showed that chemical substances with strong acid functional groups did not demonstrate genotoxicity. It was found that the genotoxicity of chemicals with functional groups of weak acids was half of that of the total amount. The genotoxicity of the neutral substance was not strong, and the genotoxicity of the weak basic substances was negligible. The zwitterions and substances with strong basic functional groups showed about half the total genotoxicity. This is the first report that has investigated the genotoxicity of zwitterions in effluents.
家用废水的各种遗传毒性物质尚未得到充分研究。本研究旨在通过固相萃取小柱根据其官能团的酸碱性质对其进行分组，然后使用umu 测试对其进行评估。用反相小柱浓缩的样品的结果表明，具有酸性官能团的物质在没有 S9 酶的情况下具有更强的遗传毒性，为 4.1-12.1ng-4-NQO/mL，而具有 S9 酶的碱性物质也表现出一定程度的毒性。使用离子交换小柱根据酸碱性质对废水进行分组的结果表明，具有强酸性官能团的化学物质没有表现出遗传毒性。研究发现，具有弱酸性官能团的化学物质的遗传毒性是总量的一半。中性物质的遗传毒性不强，弱碱性物质的遗传毒性可以忽略不计。两性离子和强碱性官能团物质的遗传毒性约为总量的一半。这是首次报道研究废水中两性离子的遗传毒性。
