Savada Camila Satie, Prosdocimi Laura, Domit Camila, Almeida Fernanda Simões de
Universidade Estadual de Londrina, Departamento de Biologia Geral, Laboratório de Genética e Ecologia Animal, 86057-970, Londrina, PR, Brazil.
Ministry of Agriculture, Livestock and Fisheries, Buenos Aires, Argentina.
Genet Mol Biol. 2021 Aug 30;44(3):e20200410. doi: 10.1590/1678-4685-GMB-2020-0410. eCollection 2021.
Mixed stocks are described for Chelonia mydas and the frequency of haplotypes in feeding areas can aid understanding of the genetic and ecological diversity, since with this information it is possible to identify the origin of the individuals. The current study aims to characterize and compare genetic diversity along the coast of Paraná with 17 other feeding areas in the Atlantic Ocean. A total of 285 samples from juveniles were DNA sequenced in the control region, resulting in the identification of 12 haplotypes, with a predominance of the CMA8 haplotype (69%) and the first registration of CMA23. For the study subjects, haplotypic and nucleotide diversity were 0.469 ± 0.032 and 0.00189 ± 0.00020, respectively, and comparisons with other feeding areas presented significant values for the majority of FST and ΦST. The results point to the importance of this region and provide evidence that over the years a mixed stock has used the region as a feeding area. This variation could be related to sea currents, climatic changes, and oceanographic characteristics that may alter the availability of food, water temperature, and the presence of turtles. The current results can be considered in conservation plans for Chelonia mydas.
描述了绿海龟的混合种群，摄食区域单倍型的频率有助于理解其遗传和生态多样性，因为利用这些信息可以确定个体的来源。本研究旨在对巴拉那海岸以及大西洋其他17个摄食区域的遗传多样性进行特征描述和比较。对285个幼龟样本的控制区进行了DNA测序，共鉴定出12种单倍型，其中CMA8单倍型占主导地位（69%），并首次记录到CMA23。研究对象的单倍型多样性和核苷酸多样性分别为0.469±0.032和0.00189±0.00020，与其他摄食区域的比较显示，大多数FST和ΦST值具有显著性。结果表明了该区域的重要性，并提供了证据表明多年来混合种群一直将该区域作为摄食区。这种变化可能与海流、气候变化以及可能改变食物供应、水温及海龟出现情况的海洋学特征有关。当前的研究结果可用于绿海龟的保护计划。
