龋齿活动和唾液供体数量的影响：在微观生物膜模型中的矿物质和微生物反应。
Influence of caries activity and number of saliva donors: mineral and microbiological responses in a microcosm biofilm model.
机构信息
Universidade Federal de Pelotas, Programa de Pós-Graduação em Odontologia, Pelotas, RS, Brasil.
Universidade Federal do Rio Grande do Sul, Departamento de Odontologia Preventiva e Social, Porto Alegre, RS, Brasil.
出版信息
J Appl Oral Sci. 2021 Sep 3;29:e20200778. doi: 10.1590/1678-7757-2020-0778. eCollection 2021.
OBJECTIVE
this study evaluated the mineral and microbiological response of biofilms originating from different types of saliva inoculum with distinct levels of caries activity.
METHODOLOGY
the biofilms grown over enamel specimens originated from saliva collected from a single donor or five donors with two distinct levels of caries activity (caries-active and caries-free) or from pooling saliva from ten donors (five caries-active and five caries-free). The percentage surface hardness change (%SHC) and microbiological counts served as outcome variables.
RESULTS
the caries activity of donors did not affect the %SHC values. Inoculum from five donors compared to a single donor showed higher %SHC values (p=0.019). Higher lactobacilli counts were observed when saliva from caries-active donors was used as the inoculum (p=0.017). Pooled saliva from both caries activity levels showed higher mutans streptococci counts (p<0.017).
CONCLUSION
Overall, pooled saliva increased the mineral response of the derived biofilms, but all the inoculum conditions formed cariogenic biofilms and caries lesions independently of caries activity.
目的
本研究评估了源自不同类型唾液接种物的生物膜的矿物质和微生物反应，这些接种物具有不同程度的龋活性。
方法
在釉质标本上生长的生物膜源自于单个供体或具有两种不同龋活性（龋活跃和无龋）的五个供体的唾液采集，或来自于十个供体（五个龋活跃和五个无龋）的唾液混合。表面硬度变化百分比（%SHC）和微生物计数作为结果变量。
结果
供体的龋活性并不影响%SHC 值。与单个供体相比，来自五个供体的接种物显示出更高的%SHC 值（p=0.019）。当使用龋活跃供体的唾液作为接种物时，观察到更高的乳杆菌计数（p=0.017）。来自两种龋活性水平的混合唾液显示出更高的变形链球菌计数（p<0.017）。
结论
总体而言，混合唾液增加了衍生生物膜的矿物质反应，但所有接种条件均独立于龋活性形成致龋生物膜和龋损。
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