通过 EGFR 和 ERK 激活分析电子烟液对小鼠脑肿瘤生长的影响。
Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.
机构信息
Department of Cancer Control and Population Health, National Cancer Center Graduate School of Cancer Science and Policy, Goyang, South Korea.
Division of Clinical Research Institute and Hospital, National Cancer Center, Goyang, Republic of Korea.
出版信息
PLoS One. 2021 Sep 8;16(9):e0256730. doi: 10.1371/journal.pone.0256730. eCollection 2021.
INTRODUCTION
Recently, electric cigarettes with liquid (e-liquid) were introduced as an alternative to tobacco smoking. They were promoted as possible cessation aids and were considered to be potentially less harmful than traditional tobacco-based cigarettes. However, there is little information on the toxicants present in e-liquids and their possible carcinogenic effects.
METHODS
Western blot analysis was performed to identify the protein levels of cancer progression related signal transducers. Patient-derived brain tumor cells (CSC2) were injected into mouse brains and tumor growth was then observed by performing magnetic resonance imaging (MRI) and hematoxylin and eosin (H&E) staining of the whole brain. Immunohistochemistry (IHC) staining and Immunofluorescence staining were performed to study the expression of pEGFR and pERK.
RESULTS
Western blotting revealed that e-liquids increased pEGFR and pERK expression in a dose dependent manner. Animal experiments revealed that the e-liquid treated group had accelerated tumor growth and poor prognosis compared to the vehicle group. Histological staining showed activation of pEGFR and pERK in the e-liquid treated group.
CONCLUSION
Our study revealed that e-liquid activates pEGFR and pERK, leading to accelerated brain tumor growth and poor prognosis.
简介
最近，推出了带液体（电子烟液）的电子烟，作为吸烟的替代品。它们被宣传为可能的戒烟辅助工具，被认为比传统的基于烟草的香烟危害更小。然而，关于电子烟液中存在的有毒物质及其可能的致癌作用的信息很少。
方法
通过 Western blot 分析来鉴定与癌症进展相关的信号转导蛋白的水平。将患者来源的脑肿瘤细胞（CSC2）注入小鼠大脑中，然后通过磁共振成像（MRI）和全脑苏木精和伊红（H&E）染色观察肿瘤生长。进行免疫组织化学（IHC）染色和免疫荧光染色来研究 pEGFR 和 pERK 的表达。
结果
Western blot 显示电子烟液以剂量依赖的方式增加了 pEGFR 和 pERK 的表达。动物实验显示，与对照组相比，电子烟液处理组的肿瘤生长加速，预后较差。组织学染色显示电子烟液处理组的 pEGFR 和 pERK 被激活。
结论
我们的研究表明，电子烟液激活了 pEGFR 和 pERK，导致脑肿瘤生长加速和预后不良。
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