Hranueli D, Pigac J, Vesligaj M
J Gen Microbiol. 1979 Oct;114(2):295-303. doi: 10.1099/00221287-114-2-295.
While searching for true lysogens among oxytetracycline-producing Streptomyces rimosus strains, free phage particles were detected and isolated from a liquid culture of S. rimosus ATCC 10970 (R7). The actinophage, designated RP2, appears to be a typical temperate DNA phage producing turbid plaques on the sensitive strain S. rimosus R6. Electron microscopic examination of RP2 lysates showed that it belongs to group B of Bradley's morphological classification. The rate of RP2 adsoprption at 28 degrees C appeared to be low. The length of the latent period was about 6 h and the average burst size about 120 phage particles. The lysogenic nature of the host-virus system described was established on the basis of the following characteristics: spontaneous lysis frequency of 2 X 10(-6) per cell, resistance to curing with phage-specific antiserum, spontaneous curing frequency of less than 0.05% and immunity to superinfection with the homologous phage. Clear-plaque mutants of RP2, which failed to lysogenize sensitive cultures, arose at a frequency of 10(-5).
在从生产土霉素的龟裂链霉菌菌株中寻找真正的溶原菌时，从龟裂链霉菌ATCC 10970（R7）的液体培养物中检测并分离出了游离噬菌体颗粒。这种放线噬菌体被命名为RP2，似乎是一种典型的温和DNA噬菌体，在敏感菌株龟裂链霉菌R6上产生浑浊噬菌斑。对RP2裂解物的电子显微镜检查表明，它属于布拉德利形态分类中的B组。RP2在28℃时的吸附速率似乎较低。潜伏期约为6小时，平均裂解量约为120个噬菌体颗粒。所描述的宿主 - 病毒系统的溶原性质是基于以下特征确定的：每个细胞的自发裂解频率为2×10⁻⁶，对用噬菌体特异性抗血清进行治愈具有抗性，自发治愈频率小于0.05%，以及对同源噬菌体的超感染具有免疫性。不能使敏感培养物溶原化的RP2透明噬菌斑突变体出现的频率为10⁻⁵。
