Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
College of Marine Sciences, Ningbo University, Ningbo, China.
J Fish Dis. 2021 Dec;44(12):2055-2066. doi: 10.1111/jfd.13520. Epub 2021 Sep 8.
The transcriptome and antibiotic resistance of Vibrio parahaemolyticus isolated from Penaeus vannamei cultured in seawater (strain HN1)and freshwater (strain SH1) ponds were studied at different salinity (2‰ and 20‰). At different salinity, 623 differentially expressed genes (DEGs) significantly upregulated and 1,559 DEGs significantly downregulated in SH1. In HN1, 466 DEGs significantly upregulated and 1,930 DEGs significantly downregulated, indicating high salinity can lead to the downregulation of most genes. In KEGG analysis, the expression of DEGs annotated to starch and sucrose metabolism pathway was higher at 2‰ salinity than at 20‰ salinity in HN1 and SH1, implying salinity affected bacterial growth mainly through this pathway. In the enrichment analysis of upregulated DEGs, two pathways (Valine, leucine, and isoleucine degradation, and Butanoate metabolism) were significantly enriched at different salinity. Antibiotic-susceptibility test discovered that SH1 isolated from P. vannamei cultured in freshwater was resistant to multiple drugs, including kanamycin, gentamicin, medemycin, and azithromycin, at a salinity of 2‰, whereas at 20‰ salinity, SH1 was not resistant to the drugs. The HN1 strain isolated from P. vannamei cultured in mariculture was resistant to polymyxin B and clindamycin at 20‰ salinity. Whereas, HN1 was intermediately susceptible to these two antibiotics at 2‰ salinity. These results indicate that the drug resistance of bacteria was affected by salinity. Furthermore, beta-lactam resistance was significantly enriched in SH1 at different salinity, and the inhibition zone of penicillin G was consistent with the results of a beta-lactam resistance pathway.
对分别在海水（HN1 株）和淡水（SH1 株）养殖池塘中养殖的凡纳滨对虾中分离得到的副溶血弧菌的转录组和抗生素耐药性进行了研究，实验分别在不同盐度（2‰和 20‰）下进行。在不同盐度下，SH1 中有 623 个差异表达基因（DEGs）显著上调，1559 个 DEGs 显著下调。在 HN1 中，有 466 个 DEGs 显著上调，1930 个 DEGs 显著下调，这表明高盐度会导致大多数基因下调。在 KEGG 分析中，在 HN1 和 SH1 中，当盐度为 2‰时，注释到淀粉和蔗糖代谢途径的 DEGs 的表达高于 20‰，这意味着盐度主要通过该途径影响细菌生长。在上调 DEGs 的富集分析中，两条途径（缬氨酸、亮氨酸和异亮氨酸降解和丁酸盐代谢）在不同盐度下显著富集。抗生素药敏试验发现，在盐度为 2‰时，从淡水养殖凡纳滨对虾中分离得到的 SH1 对包括卡那霉素、庆大霉素、麦迪霉素和阿奇霉素在内的多种药物具有耐药性，而在盐度为 20‰时，SH1 对这些药物没有耐药性。从海水养殖凡纳滨对虾中分离得到的 HN1 菌株在 20‰盐度时对多粘菌素 B 和克林霉素具有耐药性，而在 2‰盐度时，HN1 对这两种抗生素呈中度敏感。这些结果表明，细菌的耐药性受盐度的影响。此外，在不同盐度下，SH1 中β-内酰胺耐药性显著富集，青霉素 G 的抑菌圈与β-内酰胺耐药途径的结果一致。
