Karakoç Metin Deniz, Sekkin Selim
Aydın Adnan Menderes University Health Sciences Institute, Department of Pharmacology and Toxicology, Aydın, Turkey
Aydın Adnan Menderes University Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Aydın, Turkey
Turk J Pharm Sci. 2021 Sep 1;18(4):420-429. doi: 10.4274/tjps.galenos.2020.69008.
Oleuropein is the main bioactive polyphenolic compound in olive leaves, olive, and olive oil. Its anticancer, antioxidant, and antiinflammatory effects have been proven through several and studies. This study aimed to explore the effects of oleuropein on cyclophosphamideand epirubicin-induced toxicity in female rats.
Seven groups containing eight rats in each group were formed. Four cycles of 16 mg/kg/week of cyclophosphamide and 2.5 mg/kg/week of epirubicin were administered to the rats through intraperitoneal injection. Oleuropein (150 mg/kg/week) was simultaneously applied via oral gavage. The effects of oleuropein were examined with hemogram tests in whole blood samples and biochemical analysis in serum samples. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the serum samples were analyzed through enzyme-linked immunosorbent assay. Subsequently, a comet assay was performed using lymphocyte DNA. The levels of oxidant [i.e., malondialdehyde (MDA)] and antioxidants [i.e., catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD)] were measured in the heart, kidney, and liver tissues.
Oleuropein could reduce DNA damage and serum TNF-α and IL-6 levels. It also ameliorated some hemogram and biochemical parameters that deteriorated due to antineoplastic drugs. It increased the amounts of antioxidants (GSH, SOD, and CAT) and reduced the level of MDA in the heart, kidney, and liver tissues.
Oleuropein might be a beneficial agent against toxicity caused by the combination treatment of cyclophosphamide and epirubicin. Further studies should be performed to demonstrate the protective effects of oleuropein against antineoplastic induced-toxicity precisely.
橄榄苦苷是橄榄叶、橄榄和橄榄油中的主要生物活性多酚化合物。其抗癌、抗氧化和抗炎作用已通过多项研究得到证实。本研究旨在探讨橄榄苦苷对环磷酰胺和表柔比星诱导的雌性大鼠毒性的影响。
将大鼠分成七组，每组八只。通过腹腔注射对大鼠给予四个周期的环磷酰胺（16毫克/千克/周）和表柔比星（2.5毫克/千克/周）。同时通过灌胃给予橄榄苦苷（150毫克/千克/周）。通过全血样本的血常规检测和血清样本的生化分析来检查橄榄苦苷的作用。通过酶联免疫吸附测定法分析血清样本中的白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）。随后，使用淋巴细胞DNA进行彗星试验。测量心脏、肾脏和肝脏组织中氧化剂[即丙二醛（MDA）]和抗氧化剂[即过氧化氢酶（CAT）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）]的水平。
橄榄苦苷可以减少DNA损伤以及血清TNF-α和IL-6水平。它还改善了一些因抗肿瘤药物而恶化的血常规和生化参数。它增加了心脏、肾脏和肝脏组织中抗氧化剂（GSH、SOD和CAT）的含量，并降低了MDA水平。
橄榄苦苷可能是一种对抗环磷酰胺和表柔比星联合治疗所致毒性的有益药物。应进一步开展研究以准确证明橄榄苦苷对抗肿瘤诱导毒性的保护作用。
