Faculty of Pharmacy, Cyprus International University, via Mersin 10, 99258, Nicosia, Northern Cyprus, Turkey.
Biotechnology Research Center, Cyprus International University, via Mersin 10, 99258, Nicosia, Northern Cyprus, Turkey.
BMC Res Notes. 2021 Sep 8;14(1):348. doi: 10.1186/s13104-021-05762-x.
Papaver decaisnei Hochst. & Steud. Ex Elkan and Papaver glaucum Boiss. & Hausskn. growing wild in Northern Iraq have been historically used for medicinal purposes. In this study, both species were evaluated for their alkaloid content and antimicrobial activities.
Alkaloids were extracted and isolated by preparative thin-layer chromatography (TLC). Identification was carried out by comparing spectral data (UV and H-NMR) and TLC Rf values with those of authentic samples. Two alkaloids, proapaorphine-type mecambrine and aporphine-type roemerine were isolated from P. decaisnei. Two benzylisoquinoline type alkaloids papaverine (major alkaloid) and palaudine as well as aporphine-type N-methylasimilobine have been obtained in P. glaucum. Both P. glaucum and P. decaisnei extracts revealed strong antimicrobial activity on Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. Collectively these results indicate that P. glaucum and P. decaisnei are promising sources of alkaloids that could further be investigated for medicinal purposes.
生长在伊拉克北部的野生罂粟属植物已被用于医学用途。在这项研究中，评估了这两个物种的生物碱含量和抗菌活性。
通过制备性薄层色谱（TLC）提取和分离生物碱。通过比较光谱数据（UV 和 H-NMR）和 TLC Rf 值与对照品的光谱数据和 TLC Rf 值来鉴定。从罂粟属植物中分离出两种生物碱，原阿朴啡型美沙比林和阿朴啡型罗默林。从罂粟属植物中获得了两种苯并异喹啉型生物碱罂粟碱（主要生物碱）和 Palaudine 以及阿朴啡型 N-甲基asimibine。罂粟属植物和罂粟属植物提取物对铜绿假单胞菌 ATCC 27853 和粪肠球菌 ATCC 29212 均具有较强的抗菌活性。这些结果表明，罂粟属植物和罂粟属植物是具有药用前景的生物碱的有前途的来源。
