Department of Horticultural Science, Faculty of Agriculture, Lorestan University, PO Box 465, Khorramabad, Iran.
Departments of Agriculture and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Semnan, Iran.
Sci Rep. 2021 Sep 8;11(1):17839. doi: 10.1038/s41598-021-97388-z.
In this study, screening of Artemisia dracunculus accessions was investigated under water deficit based on physiological and phytochemical traits. The results clearly indicated that water deficit significantly reduced the relative water content, chlorophyll, and carotenoid contents and increased malondialdehyde, electrolyte leakage, and antioxidant activities. The responses of tarragon accessions to water deficit, however, were inconsistent. The HPLC analysis revealed the presence of chlorogenic, syringic, ferulic, vanillic, chicoric, and p-coumaric acids as major phenolic acids, while quercetin and herniarin were detected as the predominant flavonoid and coumarin compounds in the extracts. Our findings revealed that the water deficit not only increased the amounts of herniarin, luteolin, apigenin, caffeic acid, and syringic acid, but also introduced quercetin that was not present under normal conditions in Estahbanat. Nevertheless, these results were highly impacted by the accession type. The results indicated that Hamadan, Varamin and Estahbanat accessions could be introduced as tolerant accessions. Given the very different responses of tarragon accessions to water deficit and the diversity between these accessions, the findings of the present study could be an effective step in identifying and achieving homogeneous, drought-tolerant and high-yield potential accessions, and may help tarragon breeding programs as well as development of cultivation.
在这项研究中，根据生理和植物化学特性，研究了水胁迫下龙蒿种源的筛选。结果清楚地表明，水分胁迫显著降低了相对水含量、叶绿素和类胡萝卜素含量，增加了丙二醛、电解质渗漏和抗氧化活性。然而，龙蒿种源对水分胁迫的反应并不一致。HPLC 分析表明，绿原酸、丁香酸、阿魏酸、香草酸、菊苣酸和对香豆酸是主要的酚酸，而槲皮素和迷迭香酸是提取物中主要的黄酮类和香豆素化合物。我们的研究结果表明，水分胁迫不仅增加了迷迭香酸、木犀草素、芹菜素、咖啡酸和丁香酸的含量，还引入了在正常条件下不存在于 Estahbanat 的槲皮素。然而，这些结果受到种源类型的高度影响。结果表明，哈马丹、瓦拉明和埃斯塔班纳特种源可作为耐受种源引入。鉴于龙蒿种源对水分胁迫的反应非常不同，并且这些种源之间存在多样性，本研究的结果可能是识别和实现同质、耐旱和高产潜力种源的有效步骤，并可能有助于龙蒿的育种计划以及种植的发展。
