Department of Pharmaceutics, Zhoushan Women and Children Hospital, 316000, Zhoushan, Zhejiang Province, China.
Department of Teaching & Research, Zhoushan Women and Children Hospital, 316000, Zhoushan, Zhejiang Province, China.
Biomed Res Int. 2021 Aug 26;2021:1078019. doi: 10.1155/2021/1078019. eCollection 2021.
To investigate the role of curcumin in regulating pathogenesis of pulmonary arterial smooth muscle cells (PASMCs) derived from pulmonary arterial hypertension (PAH) model.
Male Sprague Dawley rats were injected with monocrotaline (MCT) to establish the PAH experimental model. The rats were divided into control group, MCT group, and curcumin group. At the end of the study, hemodynamic data were measured to determine pulmonary hypertension. Proliferation ability of PASMCs, a remodeling indicator of pulmonary artery and right ventricle, was detected. In addition, the morphology and function of mitochondria, antiglycolysis and antiproliferation pathways, and genes were also analyzed.
Curcumin may function by reversing MCT-mediated pulmonary vascular remodeling in rats. Curcumin effectively improved pulmonary vascular remodeling, promoted PASMC apoptosis, and protected mitochondrial function. In addition, curcumin treatment suppressed the PI3K/AKT pathway in PASMCs and regulated the expression of antiproliferative genes.
Curcumin can improve energy metabolism and reverse the process of PAHS. However, there were side effects of curcumin in MCT-induced rats, suggesting that the dosage should be treated with caution and its toxicological mechanism should be further studied and evaluated.
探讨姜黄素在调节肺动脉平滑肌细胞（PASMCs）发病机制中的作用，该细胞源自肺动脉高压（PAH）模型。
雄性 Sprague Dawley 大鼠注射野百合碱（MCT）建立 PAH 实验模型。大鼠分为对照组、MCT 组和姜黄素组。研究结束时，测量血流动力学数据以确定肺动脉高压。检测 PASMCs 的增殖能力，这是肺动脉和右心室重构的一个指标。此外，还分析了线粒体的形态和功能、抗糖酵解和抗增殖途径以及基因。
姜黄素可能通过逆转 MCT 介导的大鼠肺血管重塑发挥作用。姜黄素有效改善了肺血管重构，促进 PASMC 凋亡，保护线粒体功能。此外，姜黄素处理抑制了 PASMC 中的 PI3K/AKT 通路，并调节了抗增殖基因的表达。
姜黄素可改善能量代谢并逆转 PAHS 过程。然而，MCT 诱导的大鼠中姜黄素存在副作用，表明应谨慎处理剂量，并进一步研究和评估其毒理学机制。
