Nachtomi E, Farber E
Lab Invest. 1978 Mar;38(3):279-83. doi: 10.3109/00365517809108424.
Intubation of ethylene dibromide (7.5 to 10 mg. per 100 gm. of body weight) into the stomach of nonfasted Wistar rats induces DNA synthesis and cell division in the liver. The peak of DNA synthesis, as measured by 3H-methyl thymidine incorporation, was attained at or shortly after 24 hours. The mitotic waves measured with the aid of colchicine occurred at 24 to 30 hours and 48 to 54 hours after ethylene dibromide treatment. Approximately 16 per cent of liver cells entered mitosis. The increase in DNA synthesis was confirmed by autoradiography. The stimulation of liver cell mitosis occurs in nonfasted animals without apparent cell necrosis. Thus, ethylene dibromide appears to be an effective mitogen for liver under some experimental conditions. The possible relationship between the mitogenic effect at a low dosage range and necrosis at a higher range is discussed.
将二溴乙烷（每100克体重7.5至10毫克）注入未禁食的Wistar大鼠胃内，可诱导肝脏中的DNA合成和细胞分裂。通过3H-甲基胸腺嘧啶核苷掺入法测定，DNA合成的峰值在24小时或24小时后不久达到。借助秋水仙碱测量的有丝分裂波在二溴乙烷处理后24至30小时以及48至54小时出现。约16%的肝细胞进入有丝分裂。放射自显影证实了DNA合成的增加。肝细胞有丝分裂的刺激发生在未禁食的动物中，且无明显细胞坏死。因此，在某些实验条件下，二溴乙烷似乎是肝脏的一种有效促有丝分裂原。讨论了低剂量范围内的促有丝分裂作用与高剂量范围内的坏死之间的可能关系。
