Nachtomi E
Chem Biol Interact. 1980 Nov;32(3):311-9. doi: 10.1016/0009-2797(80)90098-8.
Refeeding rats treated with a single high dose of ethylene dibromide (1,2-dibromoethane, EDB) induced liver DNA synthesis. The peak of DNA synthesis, as measured by [methyl-3H]thymidine incorporation was attained after 24 h in refed rats and at 48 h in fasted ones. Fasting enhances the EDB action leading to liver cell necrosis, as shown by elevation of serum enzymes' activities, glutamic pyruvic transaminase (GPT) and sorbital dehydrogenase (SDH). A low dose of EDB administered during 2 and 3 weeks slightly enhanced the liver DNA synthesis and elevated the activity of serum enzymes. Phenobarbitone (PB) treatment of rats together with low dose of EDB during 2 weeks prevented the enzyme activity elevation and attenuated the DNA synthesis. Diethyldithiocarbamate (DDC) pretreatment potentiated the DNA synthesis in fed rats after both a small dose of EDB for 2 weeks and after a single high-dose treatment. In DDC pretreated rats, the high single dose of EDB caused biochemical perturbations in serum and liver representative of liver cell necrosis; changes in serum enzymes' activities also were noticed as early as 2 h after EDB toxication. The possible function of modulators on the mitogenic or the necrogenic action of EDB is discussed.
用单次高剂量的二溴乙烷（1,2 - 二溴乙烷，EDB）处理的大鼠再喂食后可诱导肝脏DNA合成。通过[甲基 - 3H]胸苷掺入法测定，再喂食大鼠在24小时后达到DNA合成峰值，禁食大鼠在48小时后达到峰值。禁食增强了EDB导致肝细胞坏死的作用，血清酶活性升高，如谷丙转氨酶（GPT）和山梨醇脱氢酶（SDH）所示。在2至3周内给予低剂量的EDB可轻微增强肝脏DNA合成并提高血清酶活性。在2周内用苯巴比妥（PB）处理大鼠并同时给予低剂量的EDB可防止酶活性升高并减弱DNA合成。在给予小剂量EDB 2周后以及单次高剂量处理后，二乙基二硫代氨基甲酸盐（DDC）预处理增强了喂食大鼠的DNA合成。在DDC预处理的大鼠中，高剂量单次EDB导致血清和肝脏中代表肝细胞坏死的生化紊乱；早在EDB中毒后2小时就注意到血清酶活性的变化。讨论了调节剂对EDB促有丝分裂或致坏死作用的可能功能。
