Olaris, Inc., Waltham, MA 02451, USA.
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Molecules. 2021 Aug 24;26(17):5115. doi: 10.3390/molecules26175115.
Gadolinium is a paramagnetic relaxation enhancement (PRE) agent that accelerates the relaxation of metabolite nuclei. In this study, we noted the ability of gadolinium to improve the sensitivity of two-dimensional, non-uniform sampled NMR spectral data collected from metabolomics samples. In time-equivalent experiments, the addition of gadolinium increased the mean signal intensity measurement and the signal-to-noise ratio for metabolite resonances in both standard and plasma samples. Gadolinium led to highly linear intensity measurements that correlated with metabolite concentrations. In the presence of gadolinium, we were able to detect a broad array of metabolites with a lower limit of detection and quantification in the low micromolar range. We also observed an increase in the repeatability of intensity measurements upon the addition of gadolinium. The results of this study suggest that the addition of a gadolinium-based PRE agent to metabolite samples can improve NMR-based metabolomics.
镝是一种顺磁性弛豫增强（PRE）试剂，可加速代谢物核的弛豫。在这项研究中，我们注意到镝能够提高从代谢组学样品中采集的二维、非均匀采样 NMR 谱数据的灵敏度。在时间等效实验中，添加镝可增加标准和血浆样品中代谢物共振的平均信号强度测量值和信噪比。镝导致与代谢物浓度高度线性相关的强度测量值。在镝的存在下，我们能够以低微摩尔范围的检测限和定量下限检测到广泛的代谢物。我们还观察到添加镝后强度测量值的重复性提高。这项研究的结果表明，向代谢物样品中添加基于镝的 PRE 试剂可以改善基于 NMR 的代谢组学。
