Faculty of Physics, Warsaw University of Technology, ul. Koszykowa 75, 00-662 Warszawa, Poland.
Forest Protection Department, Forest Research Institute, ul. Braci Leśnej 3, 05-090 Sękocin Stary, Poland.
Molecules. 2021 Aug 30;26(17):5272. doi: 10.3390/molecules26175272.
Identification of the presence of pathogenic oomycetes in infected plant material proved possible using an electronic nose, giving hope for a tool to assist nurseries and quarantine services. Previously, species of and intermedium have been successfully distinguished in germinated acorns of English oak L. Chemical compound analyses performed by HS-SPME/GC-MS (Headspace Solid-Phase Microextraction/Gas Chromatography-Mass Spectrometry) revealed the presence of volatile antifungal molecules produced by oak seedlings belonging to terpenes and alkanes. Compounds characteristic only of or were also found. Methylcarveol occurred when germinated acorns were infected with , while neophytadiene (isomer 2 and 3) occurred only when infected with . Moreover, isopentanol was found in acorns infected with , while in control, isopentyl vinyl ether was not observed anywhere else. Among the numerous volatile compounds, isopentanol only occurred in acorns infected with and methylcarveol in acorns infected with .
使用电子鼻可以鉴定感染植物材料中是否存在致病性卵菌，这为协助苗圃和检疫服务提供了一种工具的希望。此前，已成功区分了在英国栎 L.萌发的橡子中存在的 和 中间种。HS-SPME/GC-MS（顶空固相微萃取/气相色谱-质谱联用）进行的化合物分析表明，栎属幼苗产生了挥发性抗真菌分子，属于萜烯和烷烃。还发现了仅存在于 或 中的化合物。当感染了 时，萌发的橡子中会出现甲基橙皮油醇，而当感染了 时，只会出现新 Phytadiene（异构体 2 和 3）。此外，在感染了 的橡子中发现了异戊醇，而在对照中，在其他任何地方都没有观察到异戊基乙烯基醚。在众多挥发性化合物中，只有感染了 的橡子中会出现异戊醇，而感染了 的橡子中会出现甲基橙皮油醇。
